SplB

• Description: spore photoproduct lyase
  
  

Gene name	splB
Synonyms	spl
Essential	no
Product	spore photoproduct lyase
Function	protection of spore DNA against photodamage
Gene expression levels in SubtiExpress: splB
MW, pI	39 kDa, 8.524
Gene length, protein length	1026 bp, 342 aa
Immediate neighbours	splA, ykwB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

• Locus tag: BSU13930

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • repairs a special thymine dimer (5-thyminyl-5,6-dihydrothymine), which is commonly called spore photoproduct at the early germination phase
  • 5-thyminyl-5,6-dihydrothymine (in DNA) = 2 thymine residues (in DNA) (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):
  • contains a [4Fe-4S] cluster, uses the [4Fe-4S] cluster to reduce SAM

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P37956

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: splA-splB PubMed

• Expression browser: splB PubMed

• Sigma factor: SigG PubMed

• Regulation: expressed during sporulation in the forespore (SigG) PubMed

• Regulatory mechanism:
  • negative autoregulation by SplA PubMed

• Additional information:

Biological materials

• Mutant: BP130 (del splB::spc), available in Fabian Commichau's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: