Difference between revisions of "RecO"

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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 765 bp, 255 aa  
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 765 bp, 255 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glyQ]]'', ''[[era]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glyQ]]'', ''[[yqzL]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14457&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14457&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''

Revision as of 13:53, 3 December 2012

  • Description: mediator of RecA binding to ssDNA

Gene name recO
Synonyms yqxN, yqfI
Essential no
Product mediator of RecA binding to ssDNA
Function DNA repair/ recombination
Gene expression levels in SubtiExpress: recO
Interactions involving this protein in SubtInteract: RecO
MW, pI 29 kDa, 8.208
Gene length, protein length 765 bp, 255 aa
Immediate neighbours glyQ, yqzL
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RecO context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RecO expression.png
























Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU25280

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • provides RecA access to ssDNA during chromosomal transformation (together with DprA) PubMed
    • catalyzes annealing of SsbA or SsbA/SsbB coated ssDNAs to allow the formation of DNA duplexes with tails during plasmid transformation PubMed
  • Protein family: recO family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • cytoplasm (according to Swiss-Prot)
    • localizes to the DNA entry pole during transformation PubMed

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Tribhuwan Yadav, Begoña Carrasco, Angela R Myers, Nicholas P George, James L Keck, Juan C Alonso
Genetic recombination in Bacillus subtilis: a division of labor between two single-strand DNA-binding proteins.
Nucleic Acids Res: 2012, 40(12);5546-59
[PubMed:22373918] [WorldCat.org] [DOI] (I p)

Candela Manfredi, Yuki Suzuki, Tribhuwan Yadav, Kunio Takeyasu, Juan C Alonso
RecO-mediated DNA homology search and annealing is facilitated by SsbA.
Nucleic Acids Res: 2010, 38(20);6920-9
[PubMed:20581116] [WorldCat.org] [DOI] (I p)

Dawit Kidane, Begoña Carrasco, Candela Manfredi, Katharina Rothmaier, Silvia Ayora, Serkalem Tadesse, Juan C Alonso, Peter L Graumann
Evidence for different pathways during horizontal gene transfer in competent Bacillus subtilis cells.
PLoS Genet: 2009, 5(9);e1000630
[PubMed:19730681] [WorldCat.org] [DOI] (I p)

Candela Manfredi, Begoña Carrasco, Silvia Ayora, Juan C Alonso
Bacillus subtilis RecO nucleates RecA onto SsbA-coated single-stranded DNA.
J Biol Chem: 2008, 283(36);24837-47
[PubMed:18599486] [WorldCat.org] [DOI] (P p)

Joanna Timmins, Ingar Leiros, Sean McSweeney
Crystal structure and mutational study of RecOR provide insight into its mode of DNA binding.
EMBO J: 2007, 26(13);3260-71
[PubMed:17581636] [WorldCat.org] [DOI] (P p)