Difference between revisions of "CshA"
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
{{SubtiWiki category|[[DEAD-box RNA helicases]]}}, | {{SubtiWiki category|[[DEAD-box RNA helicases]]}}, | ||
| + | {{SubtiWiki category|[[translation]]}}, | ||
{{SubtiWiki category|[[cold stress proteins]]}}, | {{SubtiWiki category|[[cold stress proteins]]}}, | ||
{{SubtiWiki category|[[membrane proteins]]}} | {{SubtiWiki category|[[membrane proteins]]}} | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | * poor growth at low temperatures (16 to 20°C) {{PubMed|23175651}} | ||
| + | * reduced number of ribosomes {{PubMed|23175651}} | ||
=== Database entries === | === Database entries === | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cshA_511106_512641_1 cshA] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cshA_511106_512641_1 cshA] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
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=References= | =References= | ||
'''Additional publications:''' {{PubMed|20709848,21710567}} | '''Additional publications:''' {{PubMed|20709848,21710567}} | ||
| − | <pubmed>16352840,20572937 , 12399512 16861794, 21764917 ,21803996</pubmed> | + | <pubmed>16352840,20572937 , 12399512 16861794, 21764917 23175651,21803996</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 13:00, 24 November 2012
- Description: DEAD-box RNA helicase, important for adaptation to low temperatures
| Gene name | cshA |
| Synonyms | ydbR |
| Essential | no |
| Product | DEAD-box RNA helicase |
| Function | RNA helicase |
| Gene expression levels in SubtiExpress: cshA | |
| Interactions involving this protein in SubtInteract: CshA | |
| MW, pI | 57 kDa, 9.89 |
| Gene length, protein length | 1533 bp, 511 aa |
| Immediate neighbours | murF, ydbS |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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[None Expression at a glance] PubMed
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Contents
Categories containing this gene/protein
DEAD-box RNA helicases, translation, cold stress proteins, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU04580
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: RNA helicase
- Protein family: helicase C-terminal domain (according to Swiss-Prot) DEAD-box RNA helicase
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasma, colocalizes with the ribosomes PubMed, cell membrane PubMed
Database entries
- Structure:
- UniProt: P96614
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP1035 (aphA3), available in Jörg Stülke's lab
- GP1083 (cat), available in Jörg Stülke's lab
- Expression vector:
- for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, in pGP382: pGP1387, available in Jörg Stülke's lab
- for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1026 (aphA3), available in Jörg Stülke's lab
- for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP1386, available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- pGP1369 for chromosomal expression of CshA-YFP, available in Jörg Stülke's lab
- B. subtilis GP1081 cshA-gfp spc, available in Jörg Stülke's lab,
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- FLAG-tag construct:
- GP1010 (spc, based on pGP1331), available in Jörg Stülke's lab
- GP1074 (tet), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Mohamed Marahiel, Marburg University, Germany homepage
Your additional remarks
References
Additional publications: PubMed
