Difference between revisions of "DisA"

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* '''Description:''' DNA integrity scanning protein, delays sporulation in the case of chromosome damage, has diadenylate cyclase activity <br/><br/>
  
 
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|style="background:#ABCDEF;" align="center"|'''Function''' || control of sporulation initiation
 
|style="background:#ABCDEF;" align="center"|'''Function''' || control of sporulation initiation
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU00880 disA]
 
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/DisA DisA]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/DisA DisA]
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|colspan="2" | '''Genetic context''' <br/> [[Image:yacK_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yacK_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=disA_107476_108558_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:disA_expression.png|500px]]
 
 
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** ''disA'' [http://www.ncbi.nlm.nih.gov/sites/entrez/17434969 PubMed]
 
** ''disA'' [http://www.ncbi.nlm.nih.gov/sites/entrez/17434969 PubMed]
  
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=disA_107476_108558_1 disA] {{PubMed|22383849}}
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* '''[[Sigma factor]]:'''  
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* '''Sigma factor:'''  
 
 
** ''[[ctsR]]'': [[SigA]] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed], [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed1] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed2]
 
** ''[[ctsR]]'': [[SigA]] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed], [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed1] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed2]
 
** ''disA'': [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17434969 PubMed]
 
** ''disA'': [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17434969 PubMed]

Revision as of 10:13, 7 August 2012

  • Description: DNA integrity scanning protein, delays sporulation in the case of chromosome damage, has diadenylate cyclase activity

Gene name disA
Synonyms yacK
Essential no
Product DNA integrity scanning protein, has diadenylate cyclase activity
Function control of sporulation initiation
Interactions involving this protein in SubtInteract: DisA
Metabolic function and regulation of this protein in SubtiPathways:
Stress
MW, pI 40 kDa, 5.569
Gene length, protein length 1080 bp, 360 aa
Immediate neighbours radA, yacL
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YacK context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

cell division, metabolism of signalling nucleotides, sporulation/ other, general stress proteins (controlled by SigB), cell envelope stress proteins (controlled by SigM, V, W, X, Y), heat shock proteins

This gene is a member of the following regulons

CtsR regulon, SigB regulon, SigM regulon

The gene

Basic information

  • Locus tag: BSU00880

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: synthesizes the small molecule cyclic diadenosine monophosphate (c-di-AMP) PubMed
  • Protein family: disA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism: CtsR: transcription repression PubMed
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Yaara Oppenheimer-Shaanan, Ezequiel Wexselblatt, Jehoshua Katzhendler, Eylon Yavin, Sigal Ben-Yehuda
c-di-AMP reports DNA integrity during sporulation in Bacillus subtilis.
EMBO Rep: 2011, 12(6);594-601
[PubMed:21566650] [WorldCat.org] [DOI] (I p)

Ute Römling
Great times for small molecules: c-di-AMP, a second messenger candidate in Bacteria and Archaea.
Sci Signal: 2008, 1(33);pe39
[PubMed:18714086] [WorldCat.org] [DOI] (I e)

Gregor Witte, Sophia Hartung, Katharina Büttner, Karl-Peter Hopfner
Structural biochemistry of a bacterial checkpoint protein reveals diadenylate cyclase activity regulated by DNA recombination intermediates.
Mol Cell: 2008, 30(2);167-78
[PubMed:18439896] [WorldCat.org] [DOI] (I p)

Adrian J Jervis, Penny D Thackray, Chris W Houston, Malcolm J Horsburgh, Anne Moir
SigM-responsive genes of Bacillus subtilis and their promoters.
J Bacteriol: 2007, 189(12);4534-8
[PubMed:17434969] [WorldCat.org] [DOI] (P p)

Michal Bejerano-Sagie, Yaara Oppenheimer-Shaanan, Idit Berlatzky, Alex Rouvinski, Mor Meyerovich, Sigal Ben-Yehuda
A checkpoint protein that scans the chromosome for damage at the start of sporulation in Bacillus subtilis.
Cell: 2006, 125(4);679-90
[PubMed:16713562] [WorldCat.org] [DOI] (P p)

Erik Boye
DisA, a busy bee that monitors chromosome integrity.
Cell: 2006, 125(4);641-3
[PubMed:16713555] [WorldCat.org] [DOI] (P p)

Sigal Ben-Yehuda, David Z Rudner, Richard Losick
RacA, a bacterial protein that anchors chromosomes to the cell poles.
Science: 2003, 299(5606);532-6
[PubMed:12493822] [WorldCat.org] [DOI] (I p)

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)

I Derré, G Rapoport, T Msadek
CtsR, a novel regulator of stress and heat shock response, controls clp and molecular chaperone gene expression in gram-positive bacteria.
Mol Microbiol: 1999, 31(1);117-31
[PubMed:9987115] [WorldCat.org] [DOI] (P p)

E Krüger, T Msadek, M Hecker
Alternate promoters direct stress-induced transcription of the Bacillus subtilis clpC operon.
Mol Microbiol: 1996, 20(4);713-23
[PubMed:8793870] [WorldCat.org] [DOI] (P p)

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