Difference between revisions of "MecA"

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* '''Description:''' buffering protein for development, dampens transitions to spore, biofilm exopolysaccharide and competence expression , targets [[ComK]] to [[ClpC]]-[[ClpP]] degradation machine (in log Phase), inhibits the transcriptional activity of [[Spo0A]]∼P by direct interaction <br/><br/>
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|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[ComK]] degradation, regulation of competence
 
|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[ComK]] degradation, regulation of competence
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU11520 mecA]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/MecA MecA]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/MecA MecA]

Revision as of 16:45, 6 August 2012


Gene name mecA
Synonyms
Essential no
Product adaptor protein
Function control of ComK degradation, regulation of competence
Gene expression levels in SubtiExpress: mecA
Interactions involving this protein in SubtInteract: MecA
MW, pI 25 kDa, 4.209
Gene length, protein length 654 bp, 218 aa
Immediate neighbours yjbE, coiA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
MecA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MecA expression.png
























Categories containing this gene/protein

genetic competence, proteolysis

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU11520

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: mecA family (according to Swiss-Prot)
  • Paralogous protein(s): YpbH

Extended information on the protein

  • Kinetic information:
  • Domains:
    • N-terminal domain (1 ... 120): recruitment of protein substrates PubMed
    • C-terminal domain (121 ... 218): facilitates assembly of the ClpC-ClpP oligomer and acts as a degradation tag PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP813 (spc), GP814 (aphA3) both available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews: PubMed

Original Publications

Additonal publications: PubMed