Difference between revisions of "SubtInteract"

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(Important publications)
(Complexes in Information processing)
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==Complexes in [[Information processing]]==
 
==Complexes in [[Information processing]]==
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=== Initiation of [[DNA replication]]: the [[primosome]]===
 
=== [[DNA replication]]: the [[replisome]]===
 
=== [[DNA replication]]: the [[replisome]]===
 
=== [[transcription]]: [[RNA polymerase]]===
 
=== [[transcription]]: [[RNA polymerase]]===

Revision as of 16:18, 17 July 2012

Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature.


Now online: A description of SubtiWiki, SubtiPathways, and SubtInteract in the 2012 Database issue of Nucleic Acids Research


Methods to detect protein-protein interactions

  • Yeast Two Hybrid System PubMed
  • SPINE: A method to detect in vivo protein-protein interactions after cross-linking PubMed
  • in vivo detection of protein-protein interaction using DivIVA and GFP PubMed

Attention: Each technique detects only about 33% of all interactions PubMed

Visualization of protein-protein interactions in B. subtilis

Protein complexes in B. subtilis

Complexes in Cellular processes

cell wall synthesis and cell shape: the cell wall biosynthetic complex

cell division: the divisome

Complexes in Metabolism

the metabolons of glycolysis and the TCA cycle PubMed

Complexes in Information processing

Initiation of DNA replication: the primosome

DNA replication: the replisome

transcription: RNA polymerase

translation: the ribosome

synthesis of glutamyl-tRNA(Gln): the transamidosome (GatA-GatB-GatC)-GltX-trnS-Gln

RNA processing and degradation: the RNA degradosome

protein secretion: the signal recognition particle

Complexes involved in Lifestyles

general stress response: the stressosome

DNA uptake: the pseudopilus PubMed

spore germination: the germinosome

Suspected hub proteins potentially involved in a large number of interactions (as deduced from a Yeast two-hybrid analysis)


Important publications