ProB

• Description: glutamate 5-kinase
  
  

Gene name	proB
Synonyms
Essential	no
Product	glutamate 5-kinase
Function	biosynthesis of proline
Metabolic function and regulation of this protein in SubtiPathways: Pro, Stress
MW, pI	39 kDa, 6.01
Gene length, protein length	1095 bp, 365 aa
Immediate neighbours	ykkE, proA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU13120

Phenotypes of a mutant

• auxotrophic for proline PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-glutamate = ADP + L-glutamate 5-phosphate (according to Swiss-Prot)

• Protein family: PUA domain (according to Swiss-Prot)

• Paralogous protein(s): ProJ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: the enzymatic activity is feedback-inhibited by proline {PubMed|16958849}}

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P39820

• KEGG entry: [2]

• E.C. number: 2.7.2.11

Expression and regulation

• Operon: proB-proAPubMed

• Expression browser: proB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by proline limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Jeanette Brill, Tamara Hoffmann, Harald Putzer, Erhard Bremer
T-box-mediated control of the anabolic proline biosynthetic genes of Bacillus subtilis.
Microbiology (Reading): 2011, 157(Pt 4);977-987
[PubMed:21233158] [WorldCat.org] [DOI] (I p)

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Mingqing Chen, Hongbo Wei, JunWei Cao, Ruijie Liu, Youliang Wang, Congyi Zheng
Expression of Bacillus subtilis proBA genes and reduction of feedback inhibition of proline synthesis increases proline production and confers osmotolerance in transgenic Arabidopsis.
J Biochem Mol Biol: 2007, 40(3);396-403
[PubMed:17562291] [WorldCat.org] [DOI] (P p)

Mingqing Chen, Junwei Cao, Congyi Zheng, Qing Liu
Directed evolution of an artificial bifunctional enzyme, gamma-glutamyl kinase/gamma-glutamyl phosphate reductase, for improved osmotic tolerance of Escherichia coli transformants.
FEMS Microbiol Lett: 2006, 263(1);41-7
[PubMed:16958849] [WorldCat.org] [DOI] (P p)