PutC

• Description: 1-pyrroline-5-carboxylate dehydrogenase
  
  

Gene name	putC
Synonyms	ycgN
Essential	no
Product	1-pyrroline-5-carboxylate dehydrogenase
Function	proline utilization
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	56 kDa, 5.372
Gene length, protein length	1545 bp, 515 aa
Immediate neighbours	putB, putP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

CodY regulon, PutR regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU03210

Phenotypes of a mutant

• no growth with proline as single source of carbon or nitrogen PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-1-pyrroline-5-carboxylate + NAD(P)⁺ + 2 H₂O = L-glutamate + NAD(P)H (according to Swiss-Prot)

• Protein family: RocA subfamily (according to Swiss-Prot)

• Paralogous protein(s): RocA, additional similar proteins: YfmT, AldY, YcbD, GabD, GbsA, DhaS, IolA, AldX, YwdH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3RJL (1-pyrroline-5-carboxylate dehydrogenase from Bacillus licheniformis, 90% identity, 97% similarity)

• UniProt: P94391

• KEGG entry: [3]

• E.C. number: 1.5.1.12

Expression and regulation

• Operon: putB-putC-putP PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced in the presence of proline (PutR) PubMed
  • expressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • PutR: transcription activation PubMed
  • Spo0A: transcription activation PubMed
  • CodY: transcription repression, displacement of PutR PubMed

• Additional information:
  • overexpressed in a pnpA mutant PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed