Difference between revisions of "CtsR"
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=Biological materials = | =Biological materials = | ||
− | * '''Mutant:''' | + | * '''Mutant:''' ''ctsR::aphA3'' availbale from the Gerth lab |
+ | ctsRG65P::spec available from the Gerth lab | ||
− | * '''Expression vector:''' | + | * '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab |
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
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* '''two-hybrid system:''' | * '''two-hybrid system:''' | ||
− | * '''Antibody:''' | + | * '''Antibody:''' available in Gerth lab |
=Labs working on this gene/protein= | =Labs working on this gene/protein= |
Revision as of 19:55, 6 September 2011
Gene name | ctsR |
Synonyms | yacG |
Essential | no |
Product | transcription repressor |
Function | regulation of protein degradation |
Interactions involving this protein in SubtInteract: CtsR | |
Regulatory function and regulation of this protein in SubtiPathways: Stress, Phosphorelay | |
MW, pI | 17 kDa, 9.261 |
Gene length, protein length | 462 bp, 154 aa |
Immediate neighbours | rrnW-5S, mcsA |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
Categories containing this gene/protein
proteolysis, transcription factors and their control, general stress proteins (controlled by SigB), heat shock proteins, phosphoproteins
This gene is a member of the following regulons
The CtsR regulon
The gene
Basic information
- Locus tag: BSU00830
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: ctsR family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: P37568
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information: the mRNA is very stable (half-life > 15 min) PubMed
Biological materials
- Mutant: ctsR::aphA3 availbale from the Gerth lab
ctsRG65P::spec available from the Gerth lab
- Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody: available in Gerth lab
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
Additional publications: PubMed