Difference between revisions of "YvcJ"
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| − | * '''Description:''' nucleotide-binding protein <br/><br/> | + | * '''Description:''' GTPase, nucleotide-binding protein <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || GTPase |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || required for the expression of late competence genes | |style="background:#ABCDEF;" align="center"|'''Function''' || required for the expression of late competence genes | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| − | reduced competence [http://www.ncbi.nlm.nih.gov/sites/entrez/19074378 PubMed] | + | * reduced [[genetic competence]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19074378 PubMed] |
=== Database entries === | === Database entries === | ||
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=== Additional information=== | === Additional information=== | ||
| − | |||
| − | |||
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=The protein= | =The protein= | ||
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* '''Localization:''' | * '''Localization:''' | ||
| + | ** can be localized in the cell either as a helical-like pattern or as foci close to the poles and the septa depending on growth phase and on growth medium {{PubMed|21709426}} | ||
=== Database entries === | === Database entries === | ||
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=References= | =References= | ||
==Original publications== | ==Original publications== | ||
| − | <pubmed>9237995 16272399 19074378, </pubmed> | + | <pubmed>9237995 16272399 19074378, 21709426 </pubmed> |
==Publications on the ''E. coli'' homolog== | ==Publications on the ''E. coli'' homolog== | ||
<pubmed>17824929 , </pubmed> | <pubmed>17824929 , </pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 11:25, 29 June 2011
- Description: GTPase, nucleotide-binding protein
| Gene name | yvcJ |
| Synonyms | |
| Essential | no |
| Product | GTPase |
| Function | required for the expression of late competence genes |
| MW, pI | 33 kDa, 6.702 |
| Gene length, protein length | 885 bp, 295 aa |
| Immediate neighbours | yvcK, yvcI |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU34770
Phenotypes of a mutant
- reduced genetic competence PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: binds and hydrolyzes nucleotides, required for the expression of late competence genes (comK, comS) PubMed
- Protein family: UPF0042 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
- can be localized in the cell either as a helical-like pattern or as foci close to the poles and the septa depending on growth phase and on growth medium PubMed
Database entries
- Structure:
- UniProt: O06973
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation: very weak stimuation of expression by citrate and succinate PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector: pGP735 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
- Antibody:
Labs working on this gene/protein
Anne Galinier, University of Marseille, France
Your additional remarks
References
Original publications
Publications on the E. coli homolog
