SplB

• Description: spore photoproduct lyase
  
  

Gene name	splB
Synonyms	spl
Essential	no
Product	spore photoproduct lyase
Function	protection of spore DNA against photodamage
MW, pI	39 kDa, 8.524
Gene length, protein length	1026 bp, 342 aa
Immediate neighbours	splA, ykwB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

• Locus tag: BSU13930

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5-thyminyl-5,6-dihydrothymine (in DNA) = 2 thymine residues (in DNA) (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): contains an iron-sulfur cluster

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P37956

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: splA-splB PubMed

• Sigma factor: SigG PubMed

• Regulation: expressed during sporulation in the forespore (SigG) PubMed

• Regulatory mechanism:
  • negative autoregulation by SplA PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Linlin Yang, Gengjie Lin, Degang Liu, Karl J Dria, Joshua Telser, Lei Li
Probing the reaction mechanism of spore photoproduct lyase (SPL) via diastereoselectively labeled dinucleotide SP TpT substrates.
J Am Chem Soc: 2011, 133(27);10434-47
[PubMed:21671623] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

M Pedraza-Reyes, F Gutiérrez-Corona, W L Nicholson
Temporal regulation and forespore-specific expression of the spore photoproduct lyase gene by sigma-G RNA polymerase during Bacillus subtilis sporulation.
J Bacteriol: 1994, 176(13);3983-91
[PubMed:8021181] [WorldCat.org] [DOI] (P p)