Difference between revisions of "CwlD"
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<br/><br/> | <br/><br/> | ||
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| + | = [[Categories]] containing this gene/protein = | ||
| + | {{SubtiWiki category|[[germination]]}} | ||
| + | |||
| + | = This gene is a member of the following [[regulons]] = | ||
| + | {{SubtiWiki regulon|[[LexA regulon]]}}, | ||
| + | {{SubtiWiki regulon|[[SigE regulon]]}}, | ||
| + | {{SubtiWiki regulon|[[SigG regulon]]}} | ||
=The gene= | =The gene= | ||
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=The protein= | =The protein= | ||
Revision as of 15:42, 8 December 2010
- Description: N-acetylmuramoyl-L-alanine amidase
| Gene name | cwlD |
| Synonyms | |
| Essential | no |
| Product | N-acetylmuramoyl-L-alanine amidase |
| Function | spore germination |
| MW, pI | 26 kDa, 9.788 |
| Gene length, protein length | 711 bp, 237 aa |
| Immediate neighbours | ybaK, salA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
LexA regulon, SigE regulon, SigG regulon
The gene
Basic information
- Locus tag: BSU01530
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)
- Protein family: N-acetylmuramoyl-L-alanine amidase 3 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: secreted (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P50864
- KEGG entry: [3]
- E.C. number: 3.5.1.28
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Nora Au, Elke Kuester-Schoeck, Veena Mandava, Laura E Bothwell, Susan P Canny, Karen Chachu, Sierra A Colavito, Shakierah N Fuller, Eli S Groban, Laura A Hensley, Theresa C O'Brien, Amish Shah, Jessica T Tierney, Louise L Tomm, Thomas M O'Gara, Alexi I Goranov, Alan D Grossman, Charles M Lovett
Genetic composition of the Bacillus subtilis SOS system.
J Bacteriol: 2005, 187(22);7655-66
[PubMed:16267290]
[WorldCat.org]
[DOI]
(P p)
Ann E Cowan, Dennis E Koppel, Barbara Setlow, Peter Setlow
A soluble protein is immobile in dormant spores of Bacillus subtilis but is mobile in germinated spores: implications for spore dormancy.
Proc Natl Acad Sci U S A: 2003, 100(7);4209-14
[PubMed:12646705]
[WorldCat.org]
[DOI]
(P p)
D L Popham, J Meador-Parton, C E Costello, P Setlow
Spore peptidoglycan structure in a cwlD dacB double mutant of Bacillus subtilis.
J Bacteriol: 1999, 181(19);6205-9
[PubMed:10498740]
[WorldCat.org]
[DOI]
(P p)
J Sekiguchi, K Akeo, H Yamamoto, F K Khasanov, J C Alonso, A Kuroda
Nucleotide sequence and regulation of a new putative cell wall hydrolase gene, cwlD, which affects germination in Bacillus subtilis.
J Bacteriol: 1995, 177(19);5582-9
[PubMed:7559346]
[WorldCat.org]
[DOI]
(P p)
