YsxC

• Description: assembly of the 50S subunit of the ribosome
  
  

Gene name	ysxC
Synonyms	orfX
Essential	yes PubMed
Product	GTPase
Function	ribosome assembly
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	21 kDa, 9.708
Gene length, protein length	585 bp, 195 aa
Immediate neighbours	ysxD, lonA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context

The gene

Basic information

• Locus tag: BSU28190

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

translation, essential genes, membrane proteins, GTP-binding proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP

• Protein family: engB family (according to Swiss-Prot) Era/Obg family

• Paralogous protein(s): Era, Obg, YphC, YsxC, RbgA, YqeH, ThdF, YyaF, YnbA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: YsxC-RplL, YsxC-RplF, YsxC-RplC, YsxC-RplA PubMed

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure: 1SVI (complex with GDP), 1SUL

• UniProt: P38424

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: lonA-ysxC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by heat stress (CtsR) PubMed

• Regulatory mechanism:
  • CtsR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:
  • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP834, available in Stülke lab
  • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP842, available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

References

Reviews

Robert A Britton
Role of GTPases in bacterial ribosome assembly.
Annu Rev Microbiol: 2009, 63;155-76
[PubMed:19575570] [WorldCat.org] [DOI] (I p)