SpoIIIAE

• Description: component of the SpoIIIA-SpoIIQ type III secretion system residing in the forespore membrane, required for SigG activation
  
  

Gene name	spoIIIAE
Synonyms
Essential	no
Product	component of a type III secretion system
Function	activation of SigG
MW, pI	43 kDa, 6.587
Gene length, protein length	1215 bp, 405 aa
Immediate neighbours	spoIIIAF, spoIIIAD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU24390

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

protein secretion, sporulation proteins, membrane proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: SpoIIIAE-SpoIIIJ (in membrane)PubMed

• Localization: membrane protein PubMed, insertion into the membrane requires SpoIIIJ PubMed

Database entries

• Structure:

• UniProt: P49782

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoIIIAA-spoIIIAB-spoIIIAC-spoIIIAD-spoIIIAE-spoIIIAF-spoIIIAG-spoIIIAH PubMed

• Sigma factor: SigE PubMed

• Regulation: expressed during sporulation in the mother cell (SigE) PubMed, switched off by SpoIIID PubMed

• Regulatory mechanism: SpoIIID: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Richard Losick, Harvard University, Cambridge, USA homepage

Adriano Henriques, Lisbon, Portugal homepage