Dat

• Description: D-alanine aminotransferase

(Another gene coding for O6-methylguanine DNA alkyltransferase was formely known as dat, it is now called ogt)

Gene name	dat
Synonyms	yheM
Essential	no
Product	D-alanine aminotransferase
Function	peptidoglycan precursor biosynthesis
Metabolic function and regulation of this protein in SubtiPathways: Cell wall
MW, pI	31 kDa, 4.871
Gene length, protein length	846 bp, 282 aa
Immediate neighbours	yheN, nhaC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU09670

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-alanine + 2-oxoglutarate = pyruvate + D-glutamate (according to Swiss-Prot)

• Protein family: class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1G2W (E177S Mutant, Geobacillus stearothermophilus, 43% identity)

• UniProt: O07597

• KEGG entry: [2]

• E.C. number: 2.6.1.21

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Le Thi Tam, Christine Eymann, Haike Antelmann, Dirk Albrecht, Michael Hecker
Global gene expression profiling of Bacillus subtilis in response to ammonium and tryptophan starvation as revealed by transcriptome and proteome analysis.
J Mol Microbiol Biotechnol: 2007, 12(1-2);121-30
[PubMed:17183219] [WorldCat.org] [DOI] (P p)

P P Taylor, I G Fotheringham
Nucleotide sequence of the Bacillus licheniformis ATCC 10716 dat gene and comparison of the predicted amino acid sequence with those of other bacterial species.
Biochim Biophys Acta: 1997, 1350(1);38-40
[PubMed:9003455] [WorldCat.org] [DOI] (P p)