SkfA

• Description: spore killing factor
  
  

Gene name	skfA
Synonyms	ybcO
Essential	no
Product	spore killing factor
Function	killing of sister cells
MW, pI	5 kDa, 10.648
Gene length, protein length	165 bp, 55 aa
Immediate neighbours	ybcM, skfB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU01910

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: the mature SKF is cyclic 26-amino acid peptide that is posttranslationally modified with one disulfide and one cysteine thioether bridged to the alpha-position of a methionine PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: secreted (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O31422

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: skfA-skfB-skfC-skfD-skfE-skfF-skfG-skfH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by phosphate starvation (PhoP) PubMed
  • repressed by glucose (33-fold) PubMed
  • expressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed during logrithmic growth (AbrB) PubMed
  • induced by rhamnogalacturonan PubMed

• Regulatory mechanism:
  • PhoP: transcription activation PubMed
  • Spo0A: transcription activation PubMed
  • AbrB: transcription repression PubMed

• Additional information:
  • Northern blotting during during phosphate limitation showed an intense 0.25 kb skfA-specific transcript, and a weaker 6.5 kb skfA-skfB-skfC-skfD-skfE-skfF-skfG-skfH transcript.
  • The skfA transcript is stabilized by a stem-loop structure in the skfA-skfB intergenic region. PubMed
  • The mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original Publications

Wei-Ting Liu, Yu-Liang Yang, Yuquan Xu, Anne Lamsa, Nina M Haste, Jane Y Yang, Julio Ng, David Gonzalez, Craig D Ellermeier, Paul D Straight, Pavel A Pevzner, Joe Pogliano, Victor Nizet, Kit Pogliano, Pieter C Dorrestein
Imaging mass spectrometry of intraspecies metabolic exchange revealed the cannibalistic factors of Bacillus subtilis.
Proc Natl Acad Sci U S A: 2010, 107(37);16286-90
[PubMed:20805502] [WorldCat.org] [DOI] (I p)