WalR

• Description: two-component response regulator, controls cell wall metabolism
  
  

Gene name	walR
Synonyms	yycF
Essential	yes PubMed
Product	two-component response regulator
Function	control of cell wall metabolism
MW, pI	27 kDa, 4.876
Gene length, protein length	705 bp, 235 aa
Immediate neighbours	walK, trnY-Phe
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU40410

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: OmpR family of two-component transcription regulators

• Paralogous protein(s):

Genes controlled by WalR PubMed

• Activation by WalR: cwlO, lytE, ydjM, yocH, ftsA-ftsZ PubMed
• Repression by WalR: iseA, yjeA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated by WalK on an Asp residue

• Cofactor(s):

• Effectors of protein activity: phosphorylation likely affects DNA-binding activity

• Interactions: WalK-WalR

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2D1V (DNA binding domain), 2ZWM (receiver domain)

• UniProt: P37478

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: walR-walK-yycH-yycI-yycJ-yyxA PubMed

• Sigma factor:

• Regulation: expressed during vegetative growth, repressed during stationary phase PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Sarah Dubrac, Paola Bisicchia, Kevin M Devine, Tarek Msadek
A matter of life and death: cell wall homeostasis and the WalKR (YycGF) essential signal transduction pathway.
Mol Microbiol: 2008, 70(6);1307-22
[PubMed:19019149] [WorldCat.org] [DOI] (I p)

Sarah Dubrac, Tarek Msadek
Tearing down the wall: peptidoglycan metabolism and the WalK/WalR (YycG/YycF) essential two-component system.
Adv Exp Med Biol: 2008, 631;214-28
[PubMed:18792692] [WorldCat.org] [DOI] (P p)

Malcolm E Winkler, James A Hoch
Essentiality, bypass, and targeting of the YycFG (VicRK) two-component regulatory system in gram-positive bacteria.
J Bacteriol: 2008, 190(8);2645-8
[PubMed:18245295] [WorldCat.org] [DOI] (I p)