pdaC

pdaC
168

peptidoglycan deacetylase C, confers lysozyme resistance to modified cell wall peptidoglycans

Locus
BSU_12100
Molecular weight
53.67 kDa
Isoelectric point
9.1
Protein length
467 aaSequenceBlast
Gene length
1404 bpSequenceBlast
Function
cell wall modification
Product
polysaccharide deacetylase C
Essential
no
Synonyms
pdaC, yjeA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0726 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,281,128  1,282,531
Phenotypes of a mutant
sensitive to lysozyme treatment  PubMed
overexpression prevents cell wall cleavage by LytE and CwlO PubMed
The protein
Catalyzed reaction/ biological activity
deacetylates N-acetylmuramic acid (MurNAc) but not GlcNAc from peptidoglycan PubMed
Protein family
polysaccharide deacetylase family (according to UniProt)
Domains
aa 55 - 140:  DUF4163
aa 155 - 235: DUF3298
NodB homology domain (aa 278-452) (according to UniProt)
Structure
6H8L (PDB) (the C-terminal family 4 carbohydrate esterase (CE4) catalytic domain) PubMed
2C1G (PDB) (the NodB domain, from Streptococcus pneumoniae, 47% identity) PubMed
3S5T (PDB) (the DUF3298 domain, from Bacteroides fragilis, 27% identity)
O34798 (AlphaFold)
Paralogous protein(s)
YheN, (33%)
Localization
cell membrane (single pass) PubMed
Expression and Regulation
Operons
Genes
pdaC
Description
PubMed
Regulatory mechanism
WalR: repression, in walR regulon
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pdaC

2025-03-21 13:50:41

Bzhu

81

503d7be9d1b4cd984be3c844a55fd7897c2d1439

7B2BB6C3F1630A2080370377C3994774576BF132

Additional information
the mRNA is cleaved by RNase III PubMed
Biological materials
Mutant
MGNA-A355 (yjeA::erm), available at the NBRP B. subtilis, Japan
BKE12100 (pdaC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACAAAAACTCCTTCTTTC,  downstream forward: _UP4_TAAATTAGAAAAGGCTGTCC
BKK12100 (pdaC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACAAAAACTCCTTCTTTC,  downstream forward: _UP4_TAAATTAGAAAAGGCTGTCC
References
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Page visits: 5121

Time of last update: 2025-04-02 02:27:48

Author of last update: Jstuelk