SkfF

• Description: ABC transporter (permease), export of the spore killing factor
  
  

Gene name	skfF
Synonyms	ybdB
Essential	no
Product	ABC transporter (permease)
Function	export of the spore killing factor SkfA
MW, pI	51 kDa, 9.222
Gene length, protein length	1344 bp, 448 aa
Immediate neighbours	skfE, skfG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU01960

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: membrane (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: O31428

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: skfA-skfB-skfC-skfD-skfE-skfF-skfG-skfH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • Spo0A: transcription activation PubMed
  • AbrB: transcription repression PubMed

• Additional information: Northern blotting during during phosphate limitation showed an intense 0.25 kb skfA-specific transcript, and a weaker 6.5 kb skfA-skfB-skfC-skfD-skfE-skfF-skfG-skfH transcript. PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Mark A Strauch, Benjamin G Bobay, John Cavanagh, Fude Yao, Angelo Wilson, Yoann Le Breton
Abh and AbrB control of Bacillus subtilis antimicrobial gene expression.
J Bacteriol: 2007, 189(21);7720-32
[PubMed:17720793] [WorldCat.org] [DOI] (P p)

Nicholas E E Allenby, Carys A Watts, Georg Homuth, Zoltán Prágai, Anil Wipat, Alan C Ward, Colin R Harwood
Phosphate starvation induces the sporulation killing factor of Bacillus subtilis.
J Bacteriol: 2006, 188(14);5299-303
[PubMed:16816204] [WorldCat.org] [DOI] (P p)

Masaya Fujita, José Eduardo González-Pastor, Richard Losick
High- and low-threshold genes in the Spo0A regulon of Bacillus subtilis.
J Bacteriol: 2005, 187(4);1357-68
[PubMed:15687200] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

José E González-Pastor, Errett C Hobbs, Richard Losick
Cannibalism by sporulating bacteria.
Science: 2003, 301(5632);510-3
[PubMed:12817086] [WorldCat.org] [DOI] (I p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)