PfkA

• Description: is the main controling enzyme of the glycolysis. Phosphorylates fructose 6-phosphate to fructose 1,3-bisphosphate
  
  

Gene name	pfkA
Synonyms	pfk
Essential	yes
Product	6-phosphofructokinase
Function	phosphorylation of fructose 6-phosphat
MW, pI	34,1 kDa, 6.14
Gene length, protein length	957 bp, 319 amino acids
Immediate neighbours	accA, pyk
Gene sequence (+200bp)	Protein sequence
Genetic context File:GenE context.gif

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate

• Protein family: phosphofructokinase family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • 3 x nucleotide binding domain (ATP) (21–25), (154–158), (171–187)

• Modification:

• Cofactor(s): ATP

• Effectors of protein activity:

• Interactions: Pyk-PfkA

• Localization: Cytoplasm NCBI

Database entries

• Structure: Geobacillus stearothermophilus NCBI, Mutan, complex with Fructose-6-phosphate Geobacillus stearothermophilus NCBI

• Swiss prot entry: [3]

• KEGG entry: [4]

• E.C. number: [5]

Expression and regulation

• Operon: pfkA pykA

• Sigma factor:

• Regulation: induced by Glucose

• Regulatory mechanism:

• Additional information:

References

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed