Difference between revisions of "FliG"
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[flgB]]-[[flgC]]-[[fliE]]-[[fliF]]-[[fliG]]-[[fliH]]-[[fliI]]-[[fliJ]]-[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|9657996,8157612,15175317}} | + | * '''Operon:''' part of the [[fla-che operon]] (see there for regulation) |
| + | ** ''[[flgB]]-[[flgC]]-[[fliE]]-[[fliF]]-[[fliG]]-[[fliH]]-[[fliI]]-[[fliJ]]-[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|9657996,8157612,15175317}} | ||
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fliG_1694119_1695135_1 fliG] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fliG_1694119_1695135_1 fliG] {{PubMed|22383849}} | ||
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* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|9657996}}, [[SigD]] {{PubMed|9657996}} | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|9657996}}, [[SigD]] {{PubMed|9657996}} | ||
| − | * '''Regulation:''' | + | * '''Regulation:''' see [[fla-che operon]] |
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| − | * '''Regulatory mechanism:''' | + | * '''Regulatory mechanism:''' see [[fla-che operon]] |
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* '''Additional information:''' | * '''Additional information:''' | ||
Revision as of 14:10, 12 February 2015
- Description: flagellar basal-body protein, membrane anchor of the basal body, flagellar motor switch protein, physically transduces force from MotA to the rotation of FliF
| Gene name | fliG |
| Synonyms | flaA15 |
| Essential | no |
| Product | flagellar motor switch protein |
| Function | movement and chemotaxis |
| Gene expression levels in SubtiExpress: fliG | |
| Interactions involving this protein in SubtInteract: FliG | |
| MW, pI | 38 kDa, 4.46 |
| Gene length, protein length | 1014 bp, 338 aa |
| Immediate neighbours | fliF, fliH |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
motility and chemotaxis, membrane proteins
This gene is a member of the following regulons
CodY regulon, DegU regulon, SigD regulon, Spo0A regulon
The gene
Basic information
- Locus tag: BSU16220
Phenotypes of a mutant
- mucoid phenotype due to the overproduction of poly-gamma-glutamate PubMed
- no secretion of FlgM, permanent inhibition of SigD PubMed
Database entries
- BsubCyc: BSU16220
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: fliG family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity: interaction with EpsE prevents energetization of the flagellum by MotA-MotB PubMed
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- BsubCyc: BSU16220
- Structure:
- UniProt: P23448
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: part of the fla-che operon (see there for regulation)
- Regulation: see fla-che operon
- Regulatory mechanism: see fla-che operon
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium): 250 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Rebecca A Calvo, Daniel B Kearns
FlgM is secreted by the flagellar export apparatus in Bacillus subtilis.
J Bacteriol: 2015, 197(1);81-91
[PubMed:25313396]
[WorldCat.org]
[DOI]
(I p)
Serena Mordini, Cecilia Osera, Simone Marini, Francesco Scavone, Riccardo Bellazzi, Alessandro Galizzi, Cinzia Calvio
The role of SwrA, DegU and P(D3) in fla/che expression in B. subtilis.
PLoS One: 2013, 8(12);e85065
[PubMed:24386445]
[WorldCat.org]
[DOI]
(I e)
Jia Mun Chan, Sarah B Guttenplan, Daniel B Kearns
Defects in the flagellar motor increase synthesis of poly-γ-glutamate in Bacillus subtilis.
J Bacteriol: 2014, 196(4);740-53
[PubMed:24296669]
[WorldCat.org]
[DOI]
(I p)
Kris M Blair, Linda Turner, Jared T Winkelman, Howard C Berg, Daniel B Kearns
A molecular clutch disables flagella in the Bacillus subtilis biofilm.
Science: 2008, 320(5883);1636-8
[PubMed:18566286]
[WorldCat.org]
[DOI]
(I p)
Kazuo Kobayashi
Gradual activation of the response regulator DegU controls serial expression of genes for flagellum formation and biofilm formation in Bacillus subtilis.
Mol Microbiol: 2007, 66(2);395-409
[PubMed:17850253]
[WorldCat.org]
[DOI]
(P p)
H Werhane, P Lopez, M Mendel, M Zimmer, G W Ordal, L M Márquez-Magaña
The last gene of the fla/che operon in Bacillus subtilis, ylxL, is required for maximal sigmaD function.
J Bacteriol: 2004, 186(12);4025-9
[PubMed:15175317]
[WorldCat.org]
[DOI]
(P p)
Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647]
[WorldCat.org]
[DOI]
(P p)
W Estacio, S S Anna-Arriola, M Adedipe, L M Márquez-Magaña
Dual promoters are responsible for transcription initiation of the fla/che operon in Bacillus subtilis.
J Bacteriol: 1998, 180(14);3548-55
[PubMed:9657996]
[WorldCat.org]
[DOI]
(P p)
L M Márquez-Magaña, M J Chamberlin
Characterization of the sigD transcription unit of Bacillus subtilis.
J Bacteriol: 1994, 176(8);2427-34
[PubMed:8157612]
[WorldCat.org]
[DOI]
(P p)
A R Zuberi, C Ying, D S Bischoff, G W Ordal
Gene-protein relationships in the flagellar hook-basal body complex of Bacillus subtilis: sequences of the flgB, flgC, flgG, fliE and fliF genes.
Gene: 1991, 101(1);23-31
[PubMed:1905667]
[WorldCat.org]
[DOI]
(P p)
