MurF

• Description: UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine synthetase
  
  

Gene name	murF
Synonyms	ydbQ
Essential	yes PubMed
Product	UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso- 2,6- diaminopimeloyl-D-alanyl-D-alanine synthetase
Function	peptidoglycan precursor biosynthesis
Gene expression levels in SubtiExpress: murF
Metabolic function and regulation of this protein in SubtiPathways: murF
MW, pI	49 kDa, 5.337
Gene length, protein length	1371 bp, 457 aa
Immediate neighbours	ddl, cshA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, cell envelope stress proteins (controlled by SigM, V, W, X, Y), essential genes

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

• Locus tag: BSU04570

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU04570

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine (according to Swiss-Prot)

• Protein family: murCDEF family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU04570

• Structure: 1GG4 (from Escherichia coli, 32% identity, 50% similarity) PubMed

• UniProt: P96613

• KEGG entry: [2]

• E.C. number: 6.3.2.10

Expression and regulation

• Operon:

• Expression browser: murF PubMed

• Sigma factor: SigM PubMed

• Regulation:
  • expression is reduced in a SigV mutant PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 547 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 914 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421] [WorldCat.org] [DOI] (P p)

Jean van Heijenoort
Lipid intermediates in the biosynthesis of bacterial peptidoglycan.
Microbiol Mol Biol Rev: 2007, 71(4);620-35
[PubMed:18063720] [WorldCat.org] [DOI] (P p)