SasA

• Description: (p)ppGpp synthetase, small alarmone synthetase
  
  

Gene name	sasA
Synonyms	ywaC, ipa-7d
Essential	no
Product	(p)ppGpp synthetase
Function	ppGpp synthesis independent from stringent response
Gene expression levels in SubtiExpress: sasA
MW, pI	24 kDa, 6.217
Gene length, protein length	630 bp, 210 aa
Immediate neighbours	ywaD, menA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

metabolism of signalling nucleotides, cell envelope stress proteins (controlled by SigM, V, W, X, Y)

This gene is a member of the following regulons

SigM regulon SigW regulon

The gene

Basic information

• Locus tag: BSU38480

Phenotypes of a mutant

• a relA sasA sasB triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of guaB or inactivation of codY PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): SasB

Extended information on the protein

• Kinetic information:

• Domains: single ppGpp synthetase domain PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P39583

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sasA PubMed

• Expression browser: sasA PubMed

• Sigma factor: SigM PubMed SigW PubMed

• Regulation:
  • transcription is highly upregulated in response to lesions in late-acting teichoic acid synthesis genes tagB, tagD and tagF, and also in response to cell-wall-active compounds like bacitracin or vancomycin PubMed
  • expression is reduced in a SigV mutant PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: