ywaD

ywaD
168

double-zinc aminopeptidase

Locus
BSU_38470
Molecular weight
49.29 kDa
Isoelectric point
6.64
Protein length
455 aaSequenceBlast
Gene length
1368 bpSequenceBlast
Function
unknown
Product
double-zinc aminopeptidase
Essential
no
Synonyms
ywaD, ipa-8r
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2234 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,948,555  3,949,922
The protein
Catalyzed reaction/ biological activity
Release of N-terminal Arg and Lys from oligopeptides when P1' is not Pro. Also acts on arylamides of Arg and Lys (according to UniProt)
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids (according to UniProt)
Protein family
peptidase M28 family (single member, according to UniProt)
Structure
6HC6 (PDB)
6HC7 (PDB)
P25152 (AlphaFold)
Localization
extracellular (signal peptide) PubMed
Expression and Regulation
Operons
Genes
ywaD
Description
PubMed
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ywaD

2025-03-09 08:37:24

ghost

68

d7e26ec189089184a08ea54c314a273da886d187

DF22B853719D049D28EA143A9EC77622D8439210

Biological materials
Mutant
MGNA-B678 (ywaD::erm), available at the NBRP B. subtilis, Japan
BKE38470 (ywaD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAAAAATAAACCTCCCC,  downstream forward: _UP4_TAAAAAAAGACGGCACTTGG
BKK38470 (ywaD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAAAAATAAACCTCCCC,  downstream forward: _UP4_TAAAAAAAGACGGCACTTGG
References
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162B960244B0E1334F9E99E7E0F050D3312830E5

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Time of last update: 2025-03-31 17:07:58

Author of last update: Mparker