LigD

• Description: confers dry-heat resistance to dormant spores PubMed, similar to ATP-dependent DNA ligase
  
  

Gene name	ykoU
Synonyms
Essential	no
Product	unknown
Function	non-homologous end joining DNA repair
Gene expression levels in SubtiExpress: ykoU
MW, pI	70 kDa, 6.646
Gene length, protein length	1833 bp, 611 aa
Immediate neighbours	ykoT, ykoV
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins

This gene is a member of the following regulons

SigG regulon, SpoVT regulon

The gene

Basic information

• Locus tag: BSU13400

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: C-terminal DNA ligase catalytic domain linked to an N-terminal polymerase domain PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O34398

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: ykoV-ykoU PubMed

• Expression browser: ykoU PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed during sporulation in the forespore (SigG, SpoVT) PubMed

• Regulatory mechanism:
  • SpoVT: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Stewart Shuman, Michael S Glickman
Bacterial DNA repair by non-homologous end joining.
Nat Rev Microbiol: 2007, 5(11);852-61
[PubMed:17938628] [WorldCat.org] [DOI] (I p)

Original publications

Miguel de Vega
The minimal Bacillus subtilis nonhomologous end joining repair machinery.
PLoS One: 2013, 8(5);e64232
[PubMed:23691176] [WorldCat.org] [DOI] (I e)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Geoffrey R Weller, Boris Kysela, Rajat Roy, Louise M Tonkin, Elizabeth Scanlan, Marina Della, Susanne Krogh Devine, Jonathan P Day, Adam Wilkinson, Fabrizio d'Adda di Fagagna, Kevin M Devine, Richard P Bowater, Penny A Jeggo, Stephen P Jackson, Aidan J Doherty
Identification of a DNA nonhomologous end-joining complex in bacteria.
Science: 2002, 297(5587);1686-9
[PubMed:12215643] [WorldCat.org] [DOI] (I p)

G R Weller, A J Doherty
A family of DNA repair ligases in bacteria?
FEBS Lett: 2001, 505(2);340-2
[PubMed:11566200] [WorldCat.org] [DOI] (P p)

E V Koonin, Y I Wolf, A S Kondrashov, L Aravind
Bacterial homologs of the small subunit of eukaryotic DNA primase.
J Mol Microbiol Biotechnol: 2000, 2(4);509-12
[PubMed:11075926] [WorldCat.org] (P p)