RocC

• Description: amino acid permease
  
  

Gene name	rocC
Synonyms	ipa-78d
Essential	no
Product	amino acid permease
Function	arginine, ornithine and citrulline uptake
Gene expression levels in SubtiExpress: rocC
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	51 kDa, 8.41
Gene length, protein length	1410 bp, 470 aa
Immediate neighbours	ywfA, rocB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of amino acids, utilization of amino acids, membrane proteins

This gene is a member of the following regulons

AhrC regulon, CodY regulon, RocR regulon, SigL regulon

The gene

Basic information

• Locus tag: BSU37760

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:
  • secondary amino acid transporter
  • amino acid, polyamine, organocation (APC) superfamily of transporters, TC 2.A.3

• Paralogous protein(s): AapA, GabP, HutM, RocE, YbgF, YbxG, YdgF, YtnA, YvbW

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P39636

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rocA-rocB-rocC PubMed

• Expression browser: rocC PubMed

• Sigma factor: SigL PubMed

• Regulation:
  • induction by arginine (RocR, AhrC) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • RocR: transcription activation PubMed
  • AhrC: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

U Klingel, C M Miller, A K North, P G Stockley, S Baumberg
A binding site for activation by the Bacillus subtilis AhrC protein, a repressor/activator of arginine metabolism.
Mol Gen Genet: 1995, 248(3);329-40
[PubMed:7565595] [WorldCat.org] [DOI] (P p)

S Calogero, R Gardan, P Glaser, J Schweizer, G Rapoport, M Debarbouille
RocR, a novel regulatory protein controlling arginine utilization in Bacillus subtilis, belongs to the NtrC/NifA family of transcriptional activators.
J Bacteriol: 1994, 176(5);1234-41
[PubMed:8113162] [WorldCat.org] [DOI] (P p)