SpoIIGA

• Description: Pro-SigE protease
  
  

Gene name	spoIIGA
Synonyms
Essential	no
Product	Pro-SigE protease
Function	maturation of SigE
Gene expression levels in SubtiExpress: spoIIGA
Interactions involving this protein in SubtInteract: SpoIIGA
MW, pI	34 kDa, 8.552
Gene length, protein length	927 bp, 309 aa
Immediate neighbours	bpr, sigE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteolysis, sigma factors and their control, sporulation/ other, membrane proteins

This gene is a member of the following regulons

SinR regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU15310

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: peptidase U4 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • SpoIIGA protease activity is stimulated by the interaction with SpoIIR in the intermembrane space between the forespore and the mother cell PubMed

• Interactions:
  • SpoIIGA-SigE (Processing) PubMed
  • SpoIIR-SpoIIGA PubMed

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P13801

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoIIGA-sigE-sigG PubMed

• Expression browser: spoIIGA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • repression by SinR
  • Spo0A: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Additional reviews: PubMed

Original publications

Additional publications: PubMed

Steve D Seredick, Barbara M Seredick, David Baker, George B Spiegelman
An A257V mutation in the bacillus subtilis response regulator Spo0A prevents regulated expression of promoters with low-consensus binding sites.
J Bacteriol: 2009, 191(17);5489-98
[PubMed:19581368] [WorldCat.org] [DOI] (I p)

Amrita Kumar, Charles P Moran
Promoter activation by repositioning of RNA polymerase.
J Bacteriol: 2008, 190(9);3110-7
[PubMed:18296515] [WorldCat.org] [DOI] (I p)

Masaya Fujita, José Eduardo González-Pastor, Richard Losick
High- and low-threshold genes in the Spo0A regulon of Bacillus subtilis.
J Bacteriol: 2005, 187(4);1357-68
[PubMed:15687200] [WorldCat.org] [DOI] (P p)

G Schyns, C M Buckner, C P Moran
Activation of the Bacillus subtilis spoIIG promoter requires interaction of Spo0A and the sigma subunit of RNA polymerase.
J Bacteriol: 1997, 179(17);5605-8
[PubMed:9287022] [WorldCat.org] [DOI] (P p)

M Perego, P Glaser, J A Hoch
Aspartyl-phosphate phosphatases deactivate the response regulator components of the sporulation signal transduction system in Bacillus subtilis.
Mol Microbiol: 1996, 19(6);1151-7
[PubMed:8730857] [WorldCat.org] [DOI] (P p)

A E Hofmeister, A Londoño-Vallejo, E Harry, P Stragier, R Losick
Extracellular signal protein triggering the proteolytic activation of a developmental transcription factor in B. subtilis.
Cell: 1995, 83(2);219-26
[PubMed:7585939] [WorldCat.org] [DOI] (P p)

H K Peters, W G Haldenwang
Isolation of a Bacillus subtilis spoIIGA allele that suppresses processing-negative mutations in the Pro-sigma E gene (sigE).
J Bacteriol: 1994, 176(24);7763-6
[PubMed:8002606] [WorldCat.org] [DOI] (P p)

J M Baldus, B D Green, P Youngman, C P Moran
Phosphorylation of Bacillus subtilis transcription factor Spo0A stimulates transcription from the spoIIG promoter by enhancing binding to weak 0A boxes.
J Bacteriol: 1994, 176(2);296-306
[PubMed:8288522] [WorldCat.org] [DOI] (P p)

T H Bird, J K Grimsley, J A Hoch, G B Spiegelman
Phosphorylation of Spo0A activates its stimulation of in vitro transcription from the Bacillus subtilis spoIIG operon.
Mol Microbiol: 1993, 9(4);741-9
[PubMed:8231806] [WorldCat.org] [DOI] (P p)

S W Satola, J M Baldus, C P Moran
Binding of Spo0A stimulates spoIIG promoter activity in Bacillus subtilis.
J Bacteriol: 1992, 174(5);1448-53
[PubMed:1537790] [WorldCat.org] [DOI] (P p)

H K Peters, W G Haldenwang
Synthesis and fractionation properties of SpoIIGA, a protein essential for pro-sigma E processing in Bacillus subtilis.
J Bacteriol: 1991, 173(24);7821-7
[PubMed:1744037] [WorldCat.org] [DOI] (P p)

R M Jonas, E A Weaver, T J Kenney, C P Moran, W G Haldenwang
The Bacillus subtilis spoIIG operon encodes both sigma E and a gene necessary for sigma E activation.
J Bacteriol: 1988, 170(2);507-11
[PubMed:2448286] [WorldCat.org] [DOI] (P p)