PutC

• Description: 1-pyrroline-5-carboxylate dehydrogenase
  
  

Gene name	putC
Synonyms	ycgN
Essential	no
Product	1-pyrroline-5-carboxylate dehydrogenase
Function	proline utilization
Gene expression levels in SubtiExpress: putC
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	56 kDa, 5.372
Gene length, protein length	1545 bp, 515 aa
Immediate neighbours	putB, putP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

CodY regulon, PutR regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU03210

Phenotypes of a mutant

• no growth with proline as single source of carbon or nitrogen PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-1-pyrroline-5-carboxylate + NAD(P)⁺ + 2 H₂O = L-glutamate + NAD(P)H (according to Swiss-Prot)

• Protein family: RocA subfamily (according to Swiss-Prot)

• Paralogous protein(s): RocA, additional similar proteins: YfmT, AldY, YcbD, GabD, GbsA, DhaS, IolA, AldX, YwdH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3RJL (1-pyrroline-5-carboxylate dehydrogenase from Bacillus licheniformis, 90% identity, 97% similarity)

• UniProt: P94391

• KEGG entry: [3]

• E.C. number: 1.5.1.12

Expression and regulation

• Operon: putB-putC-putP PubMed

• Expression browser: putC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced in the presence of proline (PutR) PubMed
  • expressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • strongly induced in response to glucose starvation in M9 medium PubMed

• Regulatory mechanism:
  • PutR: transcription activation PubMed
  • Spo0A: transcription activation PubMed
  • CodY: transcription repression, displacement of PutR PubMed

• Additional information:
  • overexpressed in a pnpA mutant PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: