Difference between revisions of "Abn2"
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=== Additional information=== | === Additional information=== | ||
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| − | |||
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=The protein= | =The protein= | ||
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* '''Kinetic information:''' With linear-alpha-1,5-l-arabinan as the preferred substrate, the enzyme exhibited an apparent K(m) of 2.0 mg ml(-1) and V(max) of 0.25 mmol min(-1) mg(-1) at pH 7.0 and 50°C. [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed] | * '''Kinetic information:''' With linear-alpha-1,5-l-arabinan as the preferred substrate, the enzyme exhibited an apparent K(m) of 2.0 mg ml(-1) and V(max) of 0.25 mmol min(-1) mg(-1) at pH 7.0 and 50°C. [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed] | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
| − | * '''[[Localization]]:''' extracellular (signal peptide) {{PubMed|18957862,18408032}} | + | * '''[[Localization]]:''' |
| + | ** extracellular (signal peptide) {{PubMed|18957862,18408032}} | ||
=== Database entries === | === Database entries === | ||
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* '''[[Sigma factor]]:''' [[SigA]], [[SigH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed] | * '''[[Sigma factor]]:''' [[SigA]], [[SigH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed] | ||
| − | * '''Regulation:''' repressed by glucose (4.3-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed] | + | * '''Regulation:''' |
| + | ** repressed by glucose (4.3-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed] | ||
| + | ** expression is stimulated by arabinose and pectin and repressed by glucose [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed] | ||
| − | * '''Regulatory mechanism:''' [[CcpA]]: transcription repression, unknown [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed] | + | * '''Regulatory mechanism:''' |
| + | ** [[CcpA]]: transcription repression, unknown [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed] | ||
* '''Additional information:''' | * '''Additional information:''' | ||
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=References= | =References= | ||
| − | + | <pubmed>18607095, 24549757,18408032,18957862 12850135 18408032 20883454</pubmed> | |
| − | <pubmed>18607095,,18408032,18957862 12850135 18408032 </pubmed> | ||
| − | |||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 13:29, 20 February 2014
- Description: endo-1,5-alpha-L-arabinosidase
| Gene name | abn2 |
| Synonyms | yxiA |
| Essential | no |
| Product | endo-1,5-alpha-L-arabinosidase |
| Function | arabinan degradation |
| Gene expression levels in SubtiExpress: abn2 | |
| MW, pI | 52 kDa, 7.371 |
| Gene length, protein length | 1407 bp, 469 aa |
| Immediate neighbours | yxiB, yxzL |
| Gene sequence (+200bp) | Protein sequence |
| Caution: The sequence for this gene in SubtiList contains errors | |
Genetic context 
| |
Contents
Categories containing this gene/protein
utilization of specific carbon sources
This gene is a member of the following regulons
The gene
Basic information
- Locus tag:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: glycosyl hydrolase 43 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: With linear-alpha-1,5-l-arabinan as the preferred substrate, the enzyme exhibited an apparent K(m) of 2.0 mg ml(-1) and V(max) of 0.25 mmol min(-1) mg(-1) at pH 7.0 and 50°C. PubMed
- Modification:
- Effectors of protein activity:
- Localization:
- extracellular (signal peptide) PubMed
Database entries
- UniProt: P42293
- KEGG entry: [3]
- E.C. number: 3.2.1.99
Additional information
Expression and regulation
- Operon: abn2 PubMed
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Isabel de Sa-Nogueira, Lisboa, Portugal homepage
Your additional remarks
References
