Difference between revisions of "MetS"

From SubtiWiki
Jump to: navigation, search
Line 24: Line 24:
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[abrB]]'', ''[[yabD]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[abrB]]'', ''[[yabD]]''
 
|-
 
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00380 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00380 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00380 Advanced_DNA]
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00380 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00380 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00380 DNA_with_flanks]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:metS_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:metS_context.gif]]

Revision as of 09:08, 14 May 2013

  • Description: methionyl-tRNA synthetase

Gene name metS
Synonyms
Essential yes PubMed
Product methionyl-tRNA synthetase
Function translation
Gene expression levels in SubtiExpress: metS
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging
MW, pI 76 kDa, 4.958
Gene length, protein length 1992 bp, 664 aa
Immediate neighbours abrB, yabD
Sequences Protein DNA DNA_with_flanks
Genetic context
MetS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MetS expression.png




























Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

  • Locus tag: BSU00380

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met) (according to Swiss-Prot)
  • Protein family: tRNA-binding domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated on Ser-652 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1WE0 (from Thermus thermophilus hb8 mutant, 40% identity, 57% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon: metS
  • Sigma factor:
  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References