GerAB

• Description: nutrient receptor, germination response to L-alanine
  
  

Gene name	gerAB
Synonyms
Essential	no
Product	nutrient receptor
Function	germination response to L-alanine
Gene expression levels in SubtiExpress: gerAB
Interactions involving this protein in SubtInteract: GerAB
MW, pI	41 kDa, 9.601
Gene length, protein length	1095 bp, 365 aa
Immediate neighbours	gerAA, gerAC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

germination, membrane proteins

This gene is a member of the following regulons

SigF regulon, SigG regulon, SpoVT regulon

The gene

Basic information

• Locus tag: BSU33060

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GerAA-GerAB-GerAC (tentative receptor complex) PubMed
  • GerAB is part of the germinosome in the spore inner membrane PubMed

• Localization:
  • predicted to be an integral membrane protein with ten membrane-spanning helices PubMed

Database entries

• Structure:

• UniProt: P07869

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: gerAA-gerAB-gerAC PubMed

• Expression browser: gerAB PubMed

• Sigma factor: SigG PubMed, SigF PubMed

• Regulation:
  • expressed during sporulation in the forespore (SigF, SigG, SpoVT) PubMed
  • expression level depends on sporulation conditions (medim composition, temperature) PubMed

• Regulatory mechanism:
  • SpoVT: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Jing-qiao Zhang, Keren K Griffiths, Ann Cowan, Peter Setlow, Ji Yu
Expression level of Bacillus subtilis germinant receptors determines the average rate but not the heterogeneity of spore germination.
J Bacteriol: 2013, 195(8);1735-40
[PubMed:23396907] [WorldCat.org] [DOI] (I p)

Arturo Ramirez-Peralta, Pengfei Zhang, Yong-Qing Li, Peter Setlow
Effects of sporulation conditions on the germination and germination protein levels of Bacillus subtilis spores.
Appl Environ Microbiol: 2012, 78(8);2689-97
[PubMed:22327596] [WorldCat.org] [DOI] (I p)

Keren K Griffiths, Jingqiao Zhang, Ann E Cowan, Ji Yu, Peter Setlow
Germination proteins in the inner membrane of dormant Bacillus subtilis spores colocalize in a discrete cluster.
Mol Microbiol: 2011, 81(4);1061-77
[PubMed:21696470] [WorldCat.org] [DOI] (I p)

Wiyada Mongkolthanaruk, Gareth R Cooper, Julia S P Mawer, Raymond N Allan, Anne Moir
Effect of amino acid substitutions in the GerAA protein on the function of the alanine-responsive germinant receptor of Bacillus subtilis spores.
J Bacteriol: 2011, 193(9);2268-75
[PubMed:21378197] [WorldCat.org] [DOI] (I p)

Gareth R Cooper, Anne Moir
Amino acid residues in the GerAB protein important in the function and assembly of the alanine spore germination receptor of Bacillus subtilis 168.
J Bacteriol: 2011, 193(9);2261-7
[PubMed:21378181] [WorldCat.org] [DOI] (I p)

Venkata Ramana Vepachedu, Peter Setlow
Analysis of interactions between nutrient germinant receptors and SpoVA proteins of Bacillus subtilis spores.
FEMS Microbiol Lett: 2007, 274(1);42-7
[PubMed:17573930] [WorldCat.org] [DOI] (P p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Swaroopa Atluri, Katerina Ragkousi, Donna E Cortezzo, Peter Setlow
Cooperativity between different nutrient receptors in germination of spores of Bacillus subtilis and reduction of this cooperativity by alterations in the GerB receptor.
J Bacteriol: 2006, 188(1);28-36
[PubMed:16352818] [WorldCat.org] [DOI] (P p)

Takao Igarashi, Peter Setlow
Interaction between individual protein components of the GerA and GerB nutrient receptors that trigger germination of Bacillus subtilis spores.
J Bacteriol: 2005, 187(7);2513-8
[PubMed:15774895] [WorldCat.org] [DOI] (P p)

Rosa-Martha Cabrera-Martinez, Federico Tovar-Rojo, Venkata Ramana Vepachedu, Peter Setlow
Effects of overexpression of nutrient receptors on germination of spores of Bacillus subtilis.
J Bacteriol: 2003, 185(8);2457-64
[PubMed:12670969] [WorldCat.org] [DOI] (P p)

I Bagyan, J Hobot, S Cutting
A compartmentalized regulator of developmental gene expression in Bacillus subtilis.
J Bacteriol: 1996, 178(15);4500-7
[PubMed:8755877] [WorldCat.org] [DOI] (P p)

I M Feavers, J Foulkes, B Setlow, D Sun, W Nicholson, P Setlow, A Moir
The regulation of transcription of the gerA spore germination operon of Bacillus subtilis.
Mol Microbiol: 1990, 4(2);275-82
[PubMed:2110996] [WorldCat.org] [DOI] (P p)

A R Zuberi, A Moir, I M Feavers
The nucleotide sequence and gene organization of the gerA spore germination operon of Bacillus subtilis 168.
Gene: 1987, 51(1);1-11
[PubMed:3110007] [WorldCat.org] [DOI] (P p)