PolY2

• Description: translesion synthesis (TLS-) DNA polymerase Y2
  
  

Gene name	polY2
Synonyms	yqjW
Essential	no
Product	translesion synthesis (TLS-) DNA polymerase Y2
Function	UV-targeted mutagenesis
MW, pI	45 kDa, 9.532
Gene length, protein length	1236 bp, 412 aa
Immediate neighbours	yqjX, yqzH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

• Locus tag: BSU23710

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)

• Protein family: DNA polymerase type-Y family (according to Swiss-Prot)

• Paralogous protein(s): PolY1, UvrX, YozK-YobH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • PolY2-DnaN (required for untargeted mutagenesis) PubMed
  • PolA-PolY2 PubMed

• Localization:

Database entries

• Structure:

• UniProt: P54560

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: polY2-yqjX (according to DBTBS)

• Sigma factor:

• Regulation:
  • induced upon DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: GP1505 (polY2::kan), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: