CtsR

• Description: transcription repressor of class III heat shock genes (clpC operon, clpE, clpP)
  
  

Gene name	ctsR
Synonyms	yacG
Essential	no
Product	transcription repressor
Function	regulation of protein degradation
Interactions involving this protein in SubtInteract: CtsR
Regulatory function and regulation of this protein in SubtiPathways: Stress, Phosphorelay
MW, pI	17 kDa, 9.261
Gene length, protein length	462 bp, 154 aa
Immediate neighbours	rrnW-5S, mcsA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

proteolysis, transcription factors and their control, general stress proteins (controlled by SigB), heat shock proteins, phosphoproteins

This gene is a member of the following regulons

CtsR regulon, SigB regulon

The gene

Basic information

• Locus tag: BSU00830

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ctsR family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylation of a tyrosine residue by McsB PubMed
  • recently, it was reported that CtsR is phosphorylated by McsB on Arg-62 rather than on a tyrosine residue PubMed

• Cofactor(s):

• Effectors of protein activity:
  • CtsR is inactivated by heat, heat sensing requires Gly-64 PubMed
  • non-phosphorylated McsB targets CtsR for degradation PubMed
  • regulated proteolysis by ClpP/ClpC PubMed

• Interactions:
  • McsA-McsB-CtsR
  • CtsR-ClpP/ClpC (degradation of CtsR) PubMed

• Localization:

Database entries

• Structure: 3H0D (complex with a 26bp DNA duplex, from Geobacillus stearothermophilus) PubMed

• UniProt: P37568

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ctsR-mcsA-mcsB-clpC-radA-disA PubMed

• Sigma factor: SigA PubMed, SigB PubMed1 PubMed2

• Regulation:
  • induced by stress (SigB) PubMed
  • repressed at low temperatures (CtsR) PubMed

• Regulatory mechanism:
  • CtsR: transcription repression PubMed

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant: ctsR::aphA3 availbale from the Gerth lab

ctsRG65P::spec available from the Gerth lab

• Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: available in Gerth lab

References

Reviews

Alexander K W Elsholz, Ulf Gerth, Michael Hecker
Regulation of CtsR activity in low GC, Gram+ bacteria.
Adv Microb Physiol: 2010, 57;119-44
[PubMed:21078442] [WorldCat.org] [DOI] (I p)

Wolfgang Schumann
The Bacillus subtilis heat shock stimulon.
Cell Stress Chaperones: 2003, 8(3);207-17
[PubMed:14984053] [WorldCat.org] [DOI] (P p)

Original publications

Additional publications: PubMed