Difference between revisions of "SigW"

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(Categories containing this gene/protein)
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 
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* sensitive to ß-lactam antibiotics such as cefuroxime and to fosfomycin {{PubMed|22178969}}
 
=== Database entries ===
 
=== Database entries ===
  
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=References=
 
=References=
'''Additional publications:''' {{PubMed|20817771,21542858}}
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'''Additional publications:''' {{PubMed|20817771,21542858,22178969}}
 
==The [[SigW regulon]]==
 
==The [[SigW regulon]]==
 
<pubmed>9683469,16629676,17675383,11866510,9987136,10960106, </pubmed>
 
<pubmed>9683469,16629676,17675383,11866510,9987136,10960106, </pubmed>

Revision as of 18:15, 1 January 2012

  • Description: RNA polymerase ECF-type sigma factor SigW, activated by alkaline shock and by polymyxin B, vancomycin, cephalosporin C, D-cycloserine, and triton X-100

Gene name sigW
Synonyms ybbL
Essential no
Product RNA polymerase ECF-type sigma factor SigW
Function resistance against SdpC
that functions in detoxification and/or
production of antimicrobial compounds
Interactions involving this protein in SubtInteract: SigW
MW, pI 21 kDa, 8.614
Gene length, protein length 561 bp, 187 aa
Immediate neighbours trnSL-Gln2, ybbM
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SigW context.gif
This image was kindly provided by SubtiList





Categories containing this gene/protein

transcription, sigma factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y)

This gene is a member of the following regulons

AbrB regulon, SigW regulon

The SigW regulon

The gene

Basic information

  • Locus tag: BSU01730

Phenotypes of a mutant

  • sensitive to ß-lactam antibiotics such as cefuroxime and to fosfomycin PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: ECF subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: RsiW acts as antagonist of SigW (anti-SigW)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced under conditions of alkali shock or in the presence of the toxin SdpC (SigW) PubMed
    • repressed during logrithmic growth (AbrB) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

Thomas Wiegert, University of Bayreuth, Germany Homepage

Your additional remarks

References

Additional publications: PubMed

The SigW regulon

Other original publications