Difference between revisions of "SinR"

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(Extended information on the protein)
(Biological materials)
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* '''Expression vector:'''
 
* '''Expression vector:'''
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** N-terminal Strep-tag, for [[SPINE]], expression in ''B. subtilis'', in [[pGP380]]: pGP1083 , available in [[Stülke]] lab
 
          
 
          
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''

Revision as of 10:12, 2 November 2011

  • Description: transcriptional regulator of post-exponential-phase responses genes

Gene name sinR
Synonyms sin, flaD
Essential no
Product transcriptional regulator of post-exponential-phase responses genes
Function control of biofilm formation
Interactions involving this protein in SubtInteract: SinR
Metabolic function and regulation of this protein in SubtiPathways:
Biofilm, Central C-metabolism, Protein secretion
MW, pI 12 kDa, 7.177
Gene length, protein length 333 bp, 111 aa
Immediate neighbours sinI, tasA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SinR context.gif
This image was kindly provided by SubtiList





Categories containing this gene/protein

transcription factors and their control, transition state regulators, biofilm formation

This gene is a member of the following regulons

AbrB regulon, ScoC regulon, Spo0A regulon

The SinR regulon

The gene

Basic information

  • Locus tag: BSU24610

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: transcription repressor of biofilm genes, acts as co-repressor for SlrR PubMed
  • Protein family:
  • Paralogous protein(s): SlrR

Extended information on the protein

  • Kinetic information:
  • Domains:
    • DNA-binding N-terminal domain (aa 1-69) PubMed
    • SinI-binding C-terminal domain (aa 74-111) PubMed
  • Modification:
  • Cofactor(s):

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant: TMB079 sinR::spec, GP736 (tetR), available in Stülke lab
  • Expression vector:
    • N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380: pGP1083 , available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • FLAG-tag construct: GP960 (spc, based on pGP1331), available in the Stülke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Modelling of the SinI/SinR switch

Jennifer S Hallinan, Goksel Misirli, Anil Wipat
Evolutionary computation for the design of a stochastic switch for synthetic genetic circuits.
Annu Int Conf IEEE Eng Med Biol Soc: 2010, 2010;768-74
[PubMed:21095906] [WorldCat.org] [DOI] (P p)

Original publications

Additonal publications: PubMed