Difference between revisions of "SubtInteract"

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(Suspected hub proteins potentially involved in a large number of interactions)
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=== [[metabolism]]: the metabolons of glycolysis and the TCA cycle {{PubMed|19193632,20933603}}===
 
=== [[metabolism]]: the metabolons of glycolysis and the TCA cycle {{PubMed|19193632,20933603}}===
  
==Suspected hub proteins potentially involved in a large number of interactions==
+
==Suspected hub proteins potentially involved in a large number of interactions (as deduced from a Yeast two-hybrid analysis)==
 +
* [[FruA]], [SwrC], [[XhlA]], [[YhaP]], [[YhgE]], [[YkcC]], [[YqfF]], [[CsbC]], [[CssS]], [[FliZ]], [[MreD]], [[PpsC]], [[RacA]], [[Smc]], [[YclI]], [[YdeL]], [[YdgH]], [[YdbI]], [[YesS]], [[YkoT]], [[YopZ]], [[YqbD]], [[YtdP]], [[YueB]], [[YwqJ]], [[YyxA]]
 
<pubmed> 21630458</pubmed>
 
<pubmed> 21630458</pubmed>
  
 
==Important publications==
 
==Important publications==
 
<pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 18366733 10694888 2200717 2441660  20969605 15451506 </pubmed>
 
<pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 18366733 10694888 2200717 2441660  20969605 15451506 </pubmed>

Revision as of 19:49, 16 June 2011

Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature.

Methods to detect protein-protein interactions

  • Yeast Two Hybrid System PubMed
  • SPINE: A method to detect in vivo protein-protein interactions after cross-linking PubMed
  • in vivo detection of protein-protein interaction using DivIVA and GFP PubMed

Attention: Each technique detects only about 33% of all interactions PubMed

Visualization of protein-protein interactions in B. subtilis

Protein complexes in B. subtilis

DNA replication: the replisome

transcription: RNA polymerase

translation: the ribosome

synthesis of glutamyl-tRNA(Gln): the transamidosome (GatA-GatB-GatC)-GltX-trnS-Gln

RNA processing and degradation: the RNA degradosome

general stress response: the stressosome

cell division: the divisome

DNA uptake: the pseudopilus PubMed

metabolism: the metabolons of glycolysis and the TCA cycle PubMed

Suspected hub proteins potentially involved in a large number of interactions (as deduced from a Yeast two-hybrid analysis)

Elodie Marchadier, Rut Carballido-López, Sophie Brinster, Céline Fabret, Peggy Mervelet, Philippe Bessières, Marie-Françoise Noirot-Gros, Vincent Fromion, Philippe Noirot
An expanded protein-protein interaction network in Bacillus subtilis reveals a group of hubs: Exploration by an integrative approach.
Proteomics: 2011, 11(15);2981-91
[PubMed:21630458] [WorldCat.org] [DOI] (I p)


Important publications