PbpE

• Description: penicillin-binding protein PBP 4* (spore cortex)
  
  

Gene name	pbpE
Synonyms
Essential	no
Product	penicillin-binding protein PBP 4* (spore cortex)
Function	endopeptidase
MW, pI	51 kDa, 4.835
Gene length, protein length	1353 bp, 451 aa
Immediate neighbours	racX, sacB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell wall synthesis, cell wall degradation/ turnover, cell envelope stress proteins (controlled by SigM, W, X, Y)

This gene is a member of the following regulons

AbrB regulon, SigW regulon

The gene

Basic information

• Locus tag: BSU34440

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: beta-lactamase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: spore cortex

Database entries

• Structure:

• UniProt: P32959

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pbpE-racX PubMed

• Sigma factor: SigW PubMed

• Regulation:
  • repressed during logrithmic growth (AbrB) PubMed, probably indirect regulation by AbrB PubMed
  • induced by cell wall stress (SigW) PubMed

• Regulatory mechanism:

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: