DppE

• Description: dipeptide ABC transporter (dipeptide-binding protein)
  
  

Gene name	dppE
Synonyms	dciAE
Essential	no
Product	dipeptide ABC transporter (dipeptide-binding protein)
Function	uptake of dipeptides
Metabolic function and regulation of this protein in SubtiPathways: Alternative nitrogen sources
MW, pI	62 kDa, 5.523
Gene length, protein length	1647 bp, 549 aa
Immediate neighbours	dppD, ykfA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

ABC transporters, utilization of nitrogen sources other than amino acids, membrane proteins

This gene is a member of the following regulons

AbrB regulon, CodY regulon

The gene

Basic information

• Locus tag: BSU12960

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: bacterial solute-binding protein 5 family (according to Swiss-Prot)

• Paralogous protein(s): OppA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: (DppD-YkfD)-(DppB-DppC)-DppE PubMed

• Localization: attached to the cell membrane (via DppB-DppC) PubMed

Database entries

• Structure:

• UniProt: P26906

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: dppA-dppB-dppC-dppD-dppE PubMed

• Sigma factor: SigA PubMed

• Regulation: expressed postexponentially (AbrB) PubMed, repressed by CodY PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism: AbrB: transcription repression PubMed, CodY: transcription repression PubMed
  • CodY: transcription repression PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: GP1110 (spc), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: