SigE

• Description: RNA polymerase sporulation mother cell-specific (early) sigma factor SigE
  
  

Gene name	sigE
Synonyms	spoIIGB
Essential	no
Product	RNA polymerase sporulation mother cell-specific (early) sigma factor SigE
Function	transcription of sporulation genes (early mother cell)
MW, pI	27 kDa, 9.212
Gene length, protein length	717 bp, 239 aa
Immediate neighbours	spoIIGA, sigG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU15320

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

transcription, sigma factors and their control

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: RNA polymerase sigma factor, active in the mother cell

• Protein family: sigma-70 factor family (according to Swiss-Prot)

• Paralogous protein(s):

Genes controlled by SigE

dacB, pbpI, spoVD, phoB-ydhF, asnO, bdbD-bdbC, bofA, cotE, ctaE, yaaH, ybaK-cwlD, sqhC-sodF

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SigE-(RpoB-RpoC)
  • SpoIIGA-SigE (Processing)

• Localization:

Database entries

• Structure:

• UniProt: P06222

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoIIGA-sigE-sigG PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • repression by SinR
  • Spo0A: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Bill Haldenwang, San Antonio, USA
• Charles Moran, Emory University, NC, USA homepage

References

Reviews

Michiel J L de Hoon, Patrick Eichenberger, Dennis Vitkup
Hierarchical evolution of the bacterial sporulation network.
Curr Biol: 2010, 20(17);R735-45
[PubMed:20833318] [WorldCat.org] [DOI] (I p)

Original Publications

Vasant K Chary, Panagiotis Xenopoulos, Avigdor Eldar, Patrick J Piggot
Loss of compartmentalization of σ(E) activity need not prevent formation of spores by Bacillus subtilis.
J Bacteriol: 2010, 192(21);5616-24
[PubMed:20802044] [WorldCat.org] [DOI] (I p)

T Bosak, R M Losick, A Pearson
A polycyclic terpenoid that alleviates oxidative stress.
Proc Natl Acad Sci U S A: 2008, 105(18);6725-9
[PubMed:18436644] [WorldCat.org] [DOI] (I p)

Shonna M McBride, Aileen Rubio, Lei Wang, William G Haldenwang
Contributions of protein structure and gene position to the compartmentalization of the regulatory proteins sigma(E) and SpoIIE in sporulating Bacillus subtilis.
Mol Microbiol: 2005, 57(2);434-51
[PubMed:15978076] [WorldCat.org] [DOI] (P p)

Jonathan Dworkin, Richard Losick
Developmental commitment in a bacterium.
Cell: 2005, 121(3);401-9
[PubMed:15882622] [WorldCat.org] [DOI] (P p)

Masaya Fujita, José Eduardo González-Pastor, Richard Losick
High- and low-threshold genes in the Spo0A regulon of Bacillus subtilis.
J Bacteriol: 2005, 187(4);1357-68
[PubMed:15687200] [WorldCat.org] [DOI] (P p)

Jessica M Silvaggi, David L Popham, Adam Driks, Patrick Eichenberger, Richard Losick
Unmasking novel sporulation genes in Bacillus subtilis.
J Bacteriol: 2004, 186(23);8089-95
[PubMed:15547282] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

Shonna McBride, W G Haldenwang
Sporulation phenotype of a Bacillus subtilis mutant expressing an unprocessable but active sigmaE transcription factor.
J Bacteriol: 2004, 186(7);1999-2005
[PubMed:15028683] [WorldCat.org] [DOI] (P p)

Andrea Feucht, Louise Evans, Jeff Errington
Identification of sporulation genes by genome-wide analysis of the sigmaE regulon of Bacillus subtilis.
Microbiology (Reading): 2003, 149(Pt 10);3023-3034
[PubMed:14523133] [WorldCat.org] [DOI] (P p)

Ritsuko Kuwana, Satoko Yamamura, Hiromi Ikejiri, Kazuo Kobayashi, Naotake Ogasawara, Kei Asai, Yoshito Sadaie, Hiromu Takamatsu, Kazuhito Watabe
Bacillus subtilis spoVIF (yjcC) gene, involved in coat assembly and spore resistance.
Microbiology (Reading): 2003, 149(Pt 10);3011-3021
[PubMed:14523132] [WorldCat.org] [DOI] (P p)

Jingliang Ju, W G Haldenwang
Tethering of the Bacillus subtilis sigma E proprotein to the cell membrane is necessary for its processing but insufficient for its stabilization.
J Bacteriol: 2003, 185(19);5897-900
[PubMed:13129963] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922] [WorldCat.org] [DOI] (P p)

Katerina Ragkousi, Patrick Eichenberger, Christiaan van Ooij, Peter Setlow
Identification of a new gene essential for germination of Bacillus subtilis spores with Ca2+-dipicolinate.
J Bacteriol: 2003, 185(7);2315-29
[PubMed:12644503] [WorldCat.org] [DOI] (P p)

P Eichenberger, P Fawcett, R Losick
A three-protein inhibitor of polar septation during sporulation in Bacillus subtilis.
Mol Microbiol: 2001, 42(5);1147-62
[PubMed:11886548] [WorldCat.org] [DOI] (P p)

Masaya Fujita, Richard Losick
An investigation into the compartmentalization of the sporulation transcription factor sigmaE in Bacillus subtilis.
Mol Microbiol: 2002, 43(1);27-38
[PubMed:11849534] [WorldCat.org] [DOI] (P p)

P Fawcett, P Eichenberger, R Losick, P Youngman
The transcriptional profile of early to middle sporulation in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2000, 97(14);8063-8
[PubMed:10869437] [WorldCat.org] [DOI] (P p)

J Ju, W G Haldenwang
The "pro" sequence of the sporulation-specific sigma transcription factor sigma(E) directs it to the mother cell side of the sporulation septum.
J Bacteriol: 1999, 181(19);6171-5
[PubMed:10498732] [WorldCat.org] [DOI] (P p)

J Ju, T Mitchell, H Peters, W G Haldenwang
Sigma factor displacement from RNA polymerase during Bacillus subtilis sporulation.
J Bacteriol: 1999, 181(16);4969-77
[PubMed:10438769] [WorldCat.org] [DOI] (P p)

K D Price, R Losick
A four-dimensional view of assembly of a morphogenetic protein during sporulation in Bacillus subtilis.
J Bacteriol: 1999, 181(3);781-90
[PubMed:9922240] [WorldCat.org] [DOI] (P p)

J Ju, T Luo, W G Haldenwang
Forespore expression and processing of the SigE transcription factor in wild-type and mutant Bacillus subtilis.
J Bacteriol: 1998, 180(7);1673-81
[PubMed:9537362] [WorldCat.org] [DOI] (P p)

J Ju, T Luo, W G Haldenwang
Bacillus subtilis Pro-sigmaE fusion protein localizes to the forespore septum and fails to be processed when synthesized in the forespore.
J Bacteriol: 1997, 179(15);4888-93
[PubMed:9244279] [WorldCat.org] [DOI] (P p)

K Pogliano, A E Hofmeister, R Losick
Disappearance of the sigma E transcription factor from the forespore and the SpoIIE phosphatase from the mother cell contributes to establishment of cell-specific gene expression during sporulation in Bacillus subtilis.
J Bacteriol: 1997, 179(10);3331-41
[PubMed:9150232] [WorldCat.org] [DOI] (P p)

B Zhang, R A Daniel, J Errington, L Kroos
Bacillus subtilis SpoIIID protein binds to two sites in the spoVD promoter and represses transcription by sigmaE RNA polymerase.
J Bacteriol: 1997, 179(3);972-5
[PubMed:9006059] [WorldCat.org] [DOI] (P p)

H C Carlson, S Lu, L Kroos, W G Haldenwang
Exchange of precursor-specific elements between Pro-sigma E and Pro-sigma K of Bacillus subtilis.
J Bacteriol: 1996, 178(2);546-9
[PubMed:8550479] [WorldCat.org] [DOI] (P p)

A E Hofmeister, A Londoño-Vallejo, E Harry, P Stragier, R Losick
Extracellular signal protein triggering the proteolytic activation of a developmental transcription factor in B. subtilis.
Cell: 1995, 83(2);219-26
[PubMed:7585939] [WorldCat.org] [DOI] (P p)

K M Tatti, M F Shuler, C P Moran
Sequence-specific interactions between promoter DNA and the RNA polymerase sigma factor E.
J Mol Biol: 1995, 253(1);8-16
[PubMed:7473719] [WorldCat.org] [DOI] (P p)

M F Shuler, K M Tatti, K H Wade, C P Moran
A single amino acid substitution in sigma E affects its ability to bind core RNA polymerase.
J Bacteriol: 1995, 177(13);3687-94
[PubMed:7601832] [WorldCat.org] [DOI] (P p)

E J Harry, K Pogliano, R Losick
Use of immunofluorescence to visualize cell-specific gene expression during sporulation in Bacillus subtilis.
J Bacteriol: 1995, 177(12);3386-93
[PubMed:7768847] [WorldCat.org] [DOI] (P p)

H K Peters, W G Haldenwang
Isolation of a Bacillus subtilis spoIIGA allele that suppresses processing-negative mutations in the Pro-sigma E gene (sigE).
J Bacteriol: 1994, 176(24);7763-6
[PubMed:8002606] [WorldCat.org] [DOI] (P p)

P J Lewis, S R Partridge, J Errington
Sigma factors, asymmetry, and the determination of cell fate in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1994, 91(9);3849-53
[PubMed:8171000] [WorldCat.org] [DOI] (P p)

J M Baldus, B D Green, P Youngman, C P Moran
Phosphorylation of Bacillus subtilis transcription factor Spo0A stimulates transcription from the spoIIG promoter by enhancing binding to weak 0A boxes.
J Bacteriol: 1994, 176(2);296-306
[PubMed:8288522] [WorldCat.org] [DOI] (P p)

P A Levin, N Fan, E Ricca, A Driks, R Losick, S Cutting
An unusually small gene required for sporulation by Bacillus subtilis.
Mol Microbiol: 1993, 9(4);761-71
[PubMed:8231808] [WorldCat.org] [DOI] (P p)

B Beall, A Driks, R Losick, C P Moran
Cloning and characterization of a gene required for assembly of the Bacillus subtilis spore coat.
J Bacteriol: 1993, 175(6);1705-16
[PubMed:8449878] [WorldCat.org] [DOI] (P p)

C H Jones, K M Tatti, C P Moran
Effects of amino acid substitutions in the -10 binding region of sigma E from Bacillus subtilis.
J Bacteriol: 1992, 174(21);6815-21
[PubMed:1400231] [WorldCat.org] [DOI] (P p)

H K Peters, H C Carlson, W G Haldenwang
Mutational analysis of the precursor-specific region of Bacillus subtilis sigma E.
J Bacteriol: 1992, 174(14);4629-37
[PubMed:1624450] [WorldCat.org] [DOI] (P p)

C H Jones, C P Moran
Mutant sigma factor blocks transition between promoter binding and initiation of transcription.
Proc Natl Acad Sci U S A: 1992, 89(5);1958-62
[PubMed:1542693] [WorldCat.org] [DOI] (P p)

S Roels, A Driks, R Losick
Characterization of spoIVA, a sporulation gene involved in coat morphogenesis in Bacillus subtilis.
J Bacteriol: 1992, 174(2);575-85
[PubMed:1729246] [WorldCat.org] [DOI] (P p)

K M Tatti, C H Jones, C P Moran
Genetic evidence for interaction of sigma E with the spoIIID promoter in Bacillus subtilis.
J Bacteriol: 1991, 173(24);7828-33
[PubMed:1744038] [WorldCat.org] [DOI] (P p)

H K Peters, W G Haldenwang
Synthesis and fractionation properties of SpoIIGA, a protein essential for pro-sigma E processing in Bacillus subtilis.
J Bacteriol: 1991, 173(24);7821-7
[PubMed:1744037] [WorldCat.org] [DOI] (P p)

A Driks, R Losick
Compartmentalized expression of a gene under the control of sporulation transcription factor sigma E in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1991, 88(22);9934-8
[PubMed:1946462] [WorldCat.org] [DOI] (P p)

R M Jonas, S C Holt, W G Haldenwang
Effects of antibiotics on synthesis and persistence of sigma E in sporulating Bacillus subtilis.
J Bacteriol: 1990, 172(8);4616-23
[PubMed:2115871] [WorldCat.org] [DOI] (P p)

R M Jonas, H K Peters, W G Haldenwang
Phenotypes of Bacillus subtilis mutants altered in the precursor-specific region of sigma E.
J Bacteriol: 1990, 172(8);4178-86
[PubMed:2115864] [WorldCat.org] [DOI] (P p)

B Kunkel, L Kroos, H Poth, P Youngman, R Losick
Temporal and spatial control of the mother-cell regulatory gene spoIIID of Bacillus subtilis.
Genes Dev: 1989, 3(11);1735-44
[PubMed:2514119] [WorldCat.org] [DOI] (P p)

H C Carlson, W G Haldenwang
The sigma E subunit of Bacillus subtilis RNA polymerase is present in both forespore and mother cell compartments.
J Bacteriol: 1989, 171(4);2216-8
[PubMed:2495274] [WorldCat.org] [DOI] (P p)