CshA

• Description: DEAD-box RNA helicase, important for adaptation to low temperatures
  
  

Gene name	cshA
Synonyms	ydbR
Essential	no
Product	DEAD-box RNA helicase
Function	RNA helicase
MW, pI	57 kDa, 9.89
Gene length, protein length	1533 bp, 511 aa
Immediate neighbours	murF, ydbS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU04580

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: RNA helicase

• Protein family: helicase C-terminal domain (according to Swiss-Prot) DEAD-box RNA helicase

• Paralogous protein(s): CshB, DeaD, YfmL

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: CshA-CshA PubMed, CshA-CspB PubMed, CshA-PnpA PubMed, CshA-Rny PubMed, CshA-RnjA PubMed, CshA-Eno PubMed, CshA-PfkA PubMed, DeaD-CshA PubMed

• Localization: cytoplasma, colocalizes with the ribosomes PubMed, cell membrane PubMed

Database entries

• Structure:

• UniProt: P96614

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • induced by cold shock PubMed
  • constitutive, similar expression at high and low temperatures, throughout growth and in both minimal and complex medium PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP1035 (aphA3), available in Stülke lab

• Expression vector:
  • for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, in pGP382: pGP1387, available in Stülke lab
  • for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1026, available in Stülke lab
  • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP1386, available in Stülke lab

• lacZ fusion:

• GFP fusion: pGP1369 for chromosomal expression of CshA-YFP, available in Stülke lab

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• FLAG-tag construct: GP1010 (spc, based on pGP1331), available in the Stülke labs

• Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage