BacB

• Description: oxidase that catalyzes the synthesis of 2-oxo-3-(4-oxocyclohexa-2,5-dienyl)propanoic acid, a precursor to L-anticapsin
  
  

Gene name	bacB
Synonyms	ywfC, ipa-81d
Essential	no
Product	oxidase
Function	biosynthesis of the antibiotic bacilysin
MW, pI	26 kDa, 5.193
Gene length, protein length	705 bp, 235 aa
Immediate neighbours	bacC, bacA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU37730

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: synthesis of 2-oxo-3-(4-oxocyclohexa-2,5-dienyl)propanoic acid, a precursor to L-anticapsin PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3H7J PubMed

• UniProt: P39639

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: bacA-bacB-bacC-bacD-bacE-bacF PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • AbrB: transcription repression PubMed
  • ScoC: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

M Rajavel, B Gopal
Analysis of multiple crystal forms of Bacillus subtilis BacB suggests a role for a metal ion as a nucleant for crystallization.
Acta Crystallogr D Biol Crystallogr: 2010, 66(Pt 5);635-9
[PubMed:20445239] [WorldCat.org] [DOI] (I p)

Sarah A Mahlstedt, Christopher T Walsh
Investigation of anticapsin biosynthesis reveals a four-enzyme pathway to tetrahydrotyrosine in Bacillus subtilis.
Biochemistry: 2010, 49(5);912-23
[PubMed:20052993] [WorldCat.org] [DOI] (I p)

Takashi Inaoka, Guojun Wang, Kozo Ochi
ScoC regulates bacilysin production at the transcription level in Bacillus subtilis.
J Bacteriol: 2009, 191(23);7367-71
[PubMed:19801406] [WorldCat.org] [DOI] (I p)

Malligarjunan Rajavel, Ashima Mitra, Balasubramanian Gopal
Role of Bacillus subtilis BacB in the synthesis of bacilysin.
J Biol Chem: 2009, 284(46);31882-92
[PubMed:19776011] [WorldCat.org] [DOI] (I p)

Gerhard Steinborn, Mohammad-Reza Hajirezaei, Jürgen Hofemeister
bac genes for recombinant bacilysin and anticapsin production in Bacillus host strains.
Arch Microbiol: 2005, 183(2);71-9
[PubMed:15609023] [WorldCat.org] [DOI] (P p)

Takashi Inaoka, Kosaku Takahashi, Mayumi Ohnishi-Kameyama, Mitsuru Yoshida, Kozo Ochi
Guanine nucleotides guanosine 5'-diphosphate 3'-diphosphate and GTP co-operatively regulate the production of an antibiotic bacilysin in Bacillus subtilis.
J Biol Chem: 2003, 278(4);2169-76
[PubMed:12372825] [WorldCat.org] [DOI] (P p)