Difference between revisions of "CshB"
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* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| − | * '''Interactions:''' [[CshB]]-[[CspB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16352840 PubMed] | + | * '''Interactions:''' [[CshB]]-[[CspB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16352840 PubMed], [[CshB]]-[[DeaD]] {{PubMed|20572937}} |
* '''Localization:''' cytoplasma, colocalizes with the ribosomes [http://www.ncbi.nlm.nih.gov/sites/entrez/16352840 PubMed] | * '''Localization:''' cytoplasma, colocalizes with the ribosomes [http://www.ncbi.nlm.nih.gov/sites/entrez/16352840 PubMed] | ||
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* '''Regulation:''' | * '''Regulation:''' | ||
| + | ** well expressed in complex medium, poorly expressed in minimal medium {{PubMed|20572937}} | ||
| + | ** expression declines in stationary phase {{PubMed|20572937}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| Line 122: | Line 124: | ||
=References= | =References= | ||
| − | <pubmed>16352840,, 12399512, </pubmed> | + | <pubmed>16352840,, 12399512, 20572937</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 10:27, 25 June 2010
- Description: DEAD-box RNA helicase
| Gene name | cshB |
| Synonyms | yqfR |
| Essential | no |
| Product | DEAD-box RNA helicase |
| Function | RNA helicase |
| MW, pI | 49 kDa, 9.89 |
| Gene length, protein length | 1314 bp, 438 aa |
| Immediate neighbours | nfo, yqfQ |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU25140
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: RNA helicase
- Protein family: DEAD box helicase family (according to Swiss-Prot) DEAD-box RNA helicase
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasma, colocalizes with the ribosomes PubMed
Database entries
- Structure:
- UniProt: P54475
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP1002 (erm), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Mohamed Marahiel, Marburg University, Germany homepage
Your additional remarks
References
