Difference between revisions of "SkfA"
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* '''Domains:''' | * '''Domains:''' | ||
− | * '''Modification:''' | + | * '''Modification:''' the mature SKF is cyclic 26-amino acid peptide that is posttranslationally modified with one disulfide and one cysteine thioether bridged to the alpha-position of a methionine {{PubMed|20805502}} |
* '''Cofactor(s):''' | * '''Cofactor(s):''' | ||
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=References= | =References= | ||
− | <pubmed>12884008,15812018,,14651647,15687200,15687200,17720793,12850135,12817086,16816204,17449691,18840696 </pubmed> | + | <pubmed>12884008,15812018,,14651647,15687200,15687200,17720793,12850135,12817086,16816204,17449691,18840696 20805502 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 12:28, 2 September 2010
- Description: spore killing factor
Gene name | skfA |
Synonyms | ybcO |
Essential | no |
Product | spore killing factor |
Function | killing of sister cells |
MW, pI | 5 kDa, 10.648 |
Gene length, protein length | 165 bp, 55 aa |
Immediate neighbours | ybcM, skfB |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU01910
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: the mature SKF is cyclic 26-amino acid peptide that is posttranslationally modified with one disulfide and one cysteine thioether bridged to the alpha-position of a methionine PubMed
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: secreted (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O31422
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
- Northern blotting during during phosphate limitation showed an intense 0.25 kb skfA-specific transcript, and a weaker 6.5 kb skfA-skfB-skfC-skfD-skfE-skfF-skfG-skfH transcript.
- The skfA transcript is stabilized by a stem-loop structure in the skfA-skfB intergenic region. PubMed
- The mRNA is very stable (half-life > 15 min) PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References