Difference between revisions of "MurF"

From SubtiWiki
Jump to: navigation, search
(Expression and regulation)
(Database entries)
Line 85: Line 85:
 
=== Database entries ===
 
=== Database entries ===
  
* '''Structure:'''
+
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1GG4 1GG4] (from ''Escherichia coli'', 32% identity, 50% similarity) {{PubMed|11090285}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P96613 P96613]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P96613 P96613]

Revision as of 12:01, 18 February 2010

  • Description: UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine synthetase

Gene name murF
Synonyms ydbQ
Essential yes PubMed
Product UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-
2,6- diaminopimeloyl-D-alanyl-D-alanine synthetase
Function peptidoglycan precursor biosynthesis
Metabolic function and regulation of this protein in SubtiPathways:
Cell wall
MW, pI 49 kDa, 5.337
Gene length, protein length 1371 bp, 457 aa
Immediate neighbours ddl, cshA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
MurF context.gif
This image was kindly provided by SubtiList










The gene

Basic information

  • Locus tag: BSU04570

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine (according to Swiss-Prot)
  • Protein family: murCDEF family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure: 1GG4 (from Escherichia coli, 32% identity, 50% similarity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Jean van Heijenoort
Lipid intermediates in the biosynthesis of bacterial peptidoglycan.
Microbiol Mol Biol Rev: 2007, 71(4);620-35
[PubMed:18063720] [WorldCat.org] [DOI] (P p)