Difference between revisions of "SigF"
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=Expression and regulation= | =Expression and regulation= | ||
− | * '''Operon:''' ''[[dacF]]-[[spoIIAA]]-[[spoIIAB]]-[[sigF]]'' | + | * '''Operon:''' |
− | ''[[spoIIAA]]-[[spoIIAB]]-[[sigF]]'' | + | ** ''[[dacF]]-[[spoIIAA]]-[[spoIIAB]]-[[sigF]]'' |
+ | ** ''[[spoIIAA]]-[[spoIIAB]]-[[sigF]]'' | ||
− | * '''[[Sigma factor]]:''' ''[[dacF]]'': [[SigF]], [[SigG]] | + | * '''[[Sigma factor]]:''' |
− | ''[[spoIIAA]]'': [[SigH]] | + | ** ''[[dacF]]'': [[SigF]], [[SigG]] |
+ | ** ''[[spoIIAA]]'': [[SigH]] | ||
− | * '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] | + | * '''Regulation:''' |
+ | ** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] | ||
+ | ** ''[[dacF]]'': expressed during sporulation | ||
+ | ** ''[[spoIIAA]]'': expressed early during sporulation | ||
** expressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1556084,15687200 PubMed] | ** expressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1556084,15687200 PubMed] | ||
− | * '''Regulatory mechanism:''' ''[[spoIIAA]]'': activated by [[Spo0A]] and repressed by [[SinR]] | + | * '''Regulatory mechanism:''' |
+ | ** ''[[spoIIAA]]'': activated by [[Spo0A]] and repressed by [[SinR]] | ||
** [[Spo0A]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/1556084,15687200 PubMed] | ** [[Spo0A]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/1556084,15687200 PubMed] | ||
Revision as of 19:50, 18 November 2009
- Description: RNA polymerase forespore-specific (early) sigma factor SigF
Gene name | sigF |
Synonyms | spoIIAC |
Essential | no |
Product | RNA polymerase forespore-specific (early) sigma factor SigF |
Function | transcription of sporulation genes (late mother cell) |
Metabolic function and regulation of this protein in SubtiPathways: Stress | |
MW, pI | 29 kDa, 5.078 |
Gene length, protein length | 765 bp, 255 aa |
Immediate neighbours | spoVAA, spoIIAB |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU23450
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: sigma-70 factor family (according to Swiss-Prot)
- Paralogous protein(s):
Genes controlled by SigF
dacF-spoIIAA-spoIIAB-sigF, sigG, pbpF, yphA-seaA
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure: 1L0O (complex with SpoIIAB, Geobacillus stearothermophilus)
- UniProt: P07860
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
- Charles Moran, Emory University, NC, USA homepage
Your additional remarks
References