Difference between revisions of "BacF"

From SubtiWiki
Jump to: navigation, search
m (moved YwfG to BacF)
Line 1: Line 1:
* '''Description:''' similar to aspartate aminotransferase <br/><br/>
+
* '''Description:''' aspartate aminotransferase involved in bacilysin synthesis<br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ywfG''
+
|''bacF''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-85d ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywfG, ipa-85d ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
+
|style="background:#ABCDEF;" align="center"| '''Product''' || aspartate aminotransferase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
+
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of the antibiotic bacilysin
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 44 kDa, 5.347   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 44 kDa, 5.347   
Line 88: Line 88:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
+
* '''Operon:''' ''[[bacA]]-[[bacB]]-[[bacC]]-[[bacD]]-[[bacE]]-[[bacF]]'' {{PubMed|12372825}}
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
Line 95: Line 95:
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 +
** [[CodY]]: transcription repression {{PubMed|12372825}}
  
 
* '''Additional information:'''  
 
* '''Additional information:'''  
Line 117: Line 118:
  
 
=References=
 
=References=
 +
<pubmed> 12372825 </pubmed>

Revision as of 21:24, 9 July 2009

  • Description: aspartate aminotransferase involved in bacilysin synthesis

Gene name bacF
Synonyms ywfG, ipa-85d
Essential no
Product aspartate aminotransferase
Function biosynthesis of the antibiotic bacilysin
MW, pI 44 kDa, 5.347
Gene length, protein length 1197 bp, 399 aa
Immediate neighbours ywfH, bacE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YwfG context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU37690

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate (according to Swiss-Prot)
  • Protein family: class-I pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Takashi Inaoka, Kosaku Takahashi, Mayumi Ohnishi-Kameyama, Mitsuru Yoshida, Kozo Ochi
Guanine nucleotides guanosine 5'-diphosphate 3'-diphosphate and GTP co-operatively regulate the production of an antibiotic bacilysin in Bacillus subtilis.
J Biol Chem: 2003, 278(4);2169-76
[PubMed:12372825] [WorldCat.org] [DOI] (P p)