Difference between revisions of "Antibodies"

From SubtiWiki
Jump to: navigation, search
(General Antibodies)
 
(32 intermediate revisions by 4 users not shown)
Line 2: Line 2:
  
 
==General Antibodies==
 
==General Antibodies==
*anti-[[GFP]]: Dilution  
+
*anti-FLAG: Supplier: Sigma-Aldrich. Dilution: 1:10.000
*anti-[[FLAG]]
+
*anti-GFP: Supplier: MBL, Medical & Biological Laboratories. Dilution: 1:10.000
*anti-[[Strep]]
+
*anti-Strep: Supplier: IBA, Göttingen. Dilution: 1:1.000
*anti-[[His6]]
+
*anti-HIS: Supplier: Thermo. Dilution: 1:1.000
 +
*anti-rabbit-IgG-AP secondary antibody: Supplier: Promega. Dilution: 1:100.000
  
 
==Antibodies for ''Bacillus subtilis''==
 
==Antibodies for ''Bacillus subtilis''==
* anti-[[DarA]]: Dilution 1:10,000
+
* anti-[[CggR]]: Generation: By a poor rabbit @ the SeqLab Sequencing Laboratories. Dilution 1:10,000. Reference: [http://www.ncbi.nlm.nih.gov/pubmed/12634343 PubMed]
 +
* anti-[[DarA]]: Generation: SeqLab Sequencing Laboratories. Dilution 1:10,000. Reference: unpublished
 +
* anti-[[GapA]]: Generation: SeqLab Sequencing Laboratories. Dilution: 1:30.000. Reference: [http://www.ncbi.nlm.nih.gov/pubmed/12634343 PubMed] 
 +
* anti-[[GltC]]: Generation: SeqLab Sequencing Laboratories. Dilution: 1:30.000. Reference:  [http://www.ncbi.nlm.nih.gov/pubmed/17608797 PubMed]
 +
* anti-[[RocG]]: Generation: SeqLab Sequencing Laboratories. Dilution: 1:15.000. Reference:  [http://www.ncbi.nlm.nih.gov/pubmed/17608797 PubMed]
 +
* anti-[[ClpC]]: from [[Ulf Gerth]], Dilution: 1:30.000
 +
* anti-[[ClpE]]: from [[Ulf Gerth]], Dilution: 1:30.000
 +
* anti-[[ClpP]]: from [[Ulf Gerth]], Dilution: 1:100.000
 +
* anti-[[NrgB]]: Generation: SeqLab Sequencing Laboratories. {{PubMed|14600241}}
 +
* anti-[[Rny]]: from [[Jan Maarten van Dijl]], dilution 1:100.000
 +
* anti-[[Eno]]: Generation: SeqLab Sequencing Laboratories. Dilution 1:10,000. Reference: unpublished
 +
* anti-[[AccA]]: from [[xxx]], dilution 1:10.000 Reference: unpublished
 +
* anti-[[AccB]]: from [[xxx]], dilution 1:10.000 Reference: unpublished
 +
* anti-[[AccC]]: from [[xxx]], dilution 1:10.000 Reference: unpublished
 +
* anti-[[AccD]]: from [[xxx]], dilution 1:10.000 Reference: unpublished
  
 
==Antibodies for ''Listeria monocytogenes''==
 
==Antibodies for ''Listeria monocytogenes''==
* [[xxx]]: nnnnnnnnnnnnn
+
*anti-[[CdaA]]: Generation: SeqLab Sequencing Laboratories. Dilution: 1:5.000. Reference: unpublished
 +
*anti-[[PrfA]]: Generation: SeqLab Sequencing Laboratories. Dilution: 1:10.000. Reference: unpublished
  
 
==Antibodies for ''Mycoplasma pneumoniae''==
 
==Antibodies for ''Mycoplasma pneumoniae''==
*[[xxx]]: ennnnnnnnnn
+
*anti-[[GlpD]]: Generation: SeqLab Sequencing Laboratories. Dilution: 1:X0.000. Reference:  [http://www.ncbi.nlm.nih.gov/pubmed/19028882 PubMed]
 +
*anti-[[GlpK]]: Generation: SeqLab Sequencing Laboratories. Dilution: 1:X.000. Reference:  [http://www.ncbi.nlm.nih.gov/pubmed/19028882 PubMed]
 +
*anti-[[Hpr]]: Generation: SeqLab Sequencing Laboratories. Dilution: 1:5.000. Reference:  [http://www.ncbi.nlm.nih.gov/pubmed/15547265 PubMed]

Latest revision as of 11:38, 9 August 2018

Here you can find a list of available antibodies:

General Antibodies

  • anti-FLAG: Supplier: Sigma-Aldrich. Dilution: 1:10.000
  • anti-GFP: Supplier: MBL, Medical & Biological Laboratories. Dilution: 1:10.000
  • anti-Strep: Supplier: IBA, Göttingen. Dilution: 1:1.000
  • anti-HIS: Supplier: Thermo. Dilution: 1:1.000
  • anti-rabbit-IgG-AP secondary antibody: Supplier: Promega. Dilution: 1:100.000

Antibodies for Bacillus subtilis

  • anti-CggR: Generation: By a poor rabbit @ the SeqLab Sequencing Laboratories. Dilution 1:10,000. Reference: PubMed
  • anti-DarA: Generation: SeqLab Sequencing Laboratories. Dilution 1:10,000. Reference: unpublished
  • anti-GapA: Generation: SeqLab Sequencing Laboratories. Dilution: 1:30.000. Reference: PubMed
  • anti-GltC: Generation: SeqLab Sequencing Laboratories. Dilution: 1:30.000. Reference: PubMed
  • anti-RocG: Generation: SeqLab Sequencing Laboratories. Dilution: 1:15.000. Reference: PubMed
  • anti-ClpC: from Ulf Gerth, Dilution: 1:30.000
  • anti-ClpE: from Ulf Gerth, Dilution: 1:30.000
  • anti-ClpP: from Ulf Gerth, Dilution: 1:100.000
  • anti-NrgB: Generation: SeqLab Sequencing Laboratories. PubMed
  • anti-Rny: from Jan Maarten van Dijl, dilution 1:100.000
  • anti-Eno: Generation: SeqLab Sequencing Laboratories. Dilution 1:10,000. Reference: unpublished
  • anti-AccA: from xxx, dilution 1:10.000 Reference: unpublished
  • anti-AccB: from xxx, dilution 1:10.000 Reference: unpublished
  • anti-AccC: from xxx, dilution 1:10.000 Reference: unpublished
  • anti-AccD: from xxx, dilution 1:10.000 Reference: unpublished

Antibodies for Listeria monocytogenes

  • anti-CdaA: Generation: SeqLab Sequencing Laboratories. Dilution: 1:5.000. Reference: unpublished
  • anti-PrfA: Generation: SeqLab Sequencing Laboratories. Dilution: 1:10.000. Reference: unpublished

Antibodies for Mycoplasma pneumoniae

  • anti-GlpD: Generation: SeqLab Sequencing Laboratories. Dilution: 1:X0.000. Reference: PubMed
  • anti-GlpK: Generation: SeqLab Sequencing Laboratories. Dilution: 1:X.000. Reference: PubMed
  • anti-Hpr: Generation: SeqLab Sequencing Laboratories. Dilution: 1:5.000. Reference: PubMed