Difference between revisions of "FlgN"
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− | * '''Description:''' | + | * '''Description:''' flagellar filament assembly protein <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || flagellar filament assembly protein |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || flagellar filament assembly |
|- | |- | ||
− | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35420 | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35420 flgN] |
|- | |- | ||
− | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein= | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=FlgN FlgN] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 18 kDa, 8.81 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 18 kDa, 8.81 | ||
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<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
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− | |||
− | |||
− | |||
<br/><br/> | <br/><br/> | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
+ | * non-motile cells {{PubMed|24706744}} | ||
=== Database entries === | === Database entries === | ||
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=== Additional information=== | === Additional information=== | ||
− | |||
− | |||
− | |||
=The protein= | =The protein= | ||
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* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
+ | ** essential for flagellar filament assembly, export of hook-filament junction proteins [[FlgK]] and [[FlgL]] {{PubMed|24706744}} | ||
* '''Protein family:''' | * '''Protein family:''' | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
− | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
− | ** phosphorylated on Arg-60 {{PubMed|22517742}} | + | ** phosphorylated on Arg-60 {{PubMed|22517742}}, phosphorylation does not affect the properties of the protein {{PubMed|24706744}} |
− | ** phosphorylation on Tyr-49 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed] | + | ** phosphorylation on Tyr-49 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed], phosphorylation does not affect the properties of the protein {{PubMed|24706744}} |
− | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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=Expression and regulation= | =Expression and regulation= | ||
* '''Operon:''' | * '''Operon:''' | ||
− | ** ''[[comFA]]-[[comFB]]-[[comFC]]-[[yvyF]]-[[flgM]]-[[ | + | ** ''[[comFA]]-[[comFB]]-[[comFC]]-[[yvyF]]-[[flgM]]-[[flgN]]-[[flgK]]-[[flgL]]'' {{PubMed|8412657}} |
− | ** ''[[yvyF]]-[[flgM]]-[[ | + | ** ''[[yvyF]]-[[flgM]]-[[flgN]]-[[flgK]]-[[flgL]]'' {{PubMed|8045879}} |
− | ** ''[[flgM]]-[[ | + | ** ''[[flgM]]-[[flgN]]-[[flgK]]-[[flgL]]'' {{PubMed|21736639}} |
− | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yvyG_3639787_3640269_-1 | + | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yvyG_3639787_3640269_-1 flgN] {{PubMed|22383849}} |
− | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
** ''[[comFA]]'': [[SigA]] {{PubMed|8412657}} | ** ''[[comFA]]'': [[SigA]] {{PubMed|8412657}} | ||
** ''[[yvyF]]'': [[SigD]] {{PubMed|8045879}} | ** ''[[yvyF]]'': [[SigD]] {{PubMed|8045879}} | ||
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=References= | =References= | ||
− | + | <pubmed>8412657 8045879 17218307, 15817382 22517742 24706744 21736639 24728941 </pubmed> | |
− | <pubmed>8412657 8045879 17218307, 15817382 22517742</pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Latest revision as of 07:13, 15 April 2014
- Description: flagellar filament assembly protein
Gene name | yvyG |
Synonyms | yviC |
Essential | no |
Product | flagellar filament assembly protein |
Function | flagellar filament assembly |
Gene expression levels in SubtiExpress: flgN | |
Interactions involving this protein in SubtInteract: FlgN | |
MW, pI | 18 kDa, 8.81 |
Gene length, protein length | 480 bp, 160 aa |
Immediate neighbours | flgK, flgM |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
motility and chemotaxis, phosphoproteins
This gene is a member of the following regulons
ComK regulon, DegU regulon, SigD regulon
The gene
Basic information
- Locus tag: BSU35420
Phenotypes of a mutant
- non-motile cells PubMed
Database entries
- BsubCyc: BSU35420
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
Database entries
- BsubCyc: BSU35420
- Structure:
- UniProt: P39808
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Lei Shi, Boyang Ji, Lorena Kolar-Znika, Ana Boskovic, Fanny Jadeau, Christophe Combet, Christophe Grangeasse, Damjan Franjevic, Emmanuel Talla, Ivan Mijakovic
Evolution of bacterial protein-tyrosine kinases and their relaxed specificity toward substrates.
Genome Biol Evol: 2014, 6(4);800-17
[PubMed:24728941]
[WorldCat.org]
[DOI]
(I p)
Lynne S Cairns, Victoria L Marlow, Taryn B Kiley, Christopher Birchall, Adam Ostrowski, Phillip D Aldridge, Nicola R Stanley-Wall
FlgN is required for flagellum-based motility by Bacillus subtilis.
J Bacteriol: 2014, 196(12);2216-26
[PubMed:24706744]
[WorldCat.org]
[DOI]
(I p)
Alexander K W Elsholz, Kürsad Turgay, Stephan Michalik, Bernd Hessling, Katrin Gronau, Dan Oertel, Ulrike Mäder, Jörg Bernhardt, Dörte Becher, Michael Hecker, Ulf Gerth
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Proc Natl Acad Sci U S A: 2012, 109(19);7451-6
[PubMed:22517742]
[WorldCat.org]
[DOI]
(I p)
Yi-Huang Hsueh, Loralyn M Cozy, Lok-To Sham, Rebecca A Calvo, Alina D Gutu, Malcolm E Winkler, Daniel B Kearns
DegU-phosphate activates expression of the anti-sigma factor FlgM in Bacillus subtilis.
Mol Microbiol: 2011, 81(4);1092-108
[PubMed:21736639]
[WorldCat.org]
[DOI]
(I p)
Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307]
[WorldCat.org]
[DOI]
(P p)
Mark J Pallen, Charles W Penn, Roy R Chaudhuri
Bacterial flagellar diversity in the post-genomic era.
Trends Microbiol: 2005, 13(4);143-9
[PubMed:15817382]
[WorldCat.org]
[DOI]
(P p)
D B Mirel, P Lauer, M J Chamberlin
Identification of flagellar synthesis regulatory and structural genes in a sigma D-dependent operon of Bacillus subtilis.
J Bacteriol: 1994, 176(15);4492-500
[PubMed:8045879]
[WorldCat.org]
[DOI]
(P p)
J A Londoño-Vallejo, D Dubnau
comF, a Bacillus subtilis late competence locus, encodes a protein similar to ATP-dependent RNA/DNA helicases.
Mol Microbiol: 1993, 9(1);119-31
[PubMed:8412657]
[WorldCat.org]
[DOI]
(P p)