Difference between revisions of "GltA"
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|style="background:#ABCDEF;" align="center"|'''Function''' || glutamate biosynthesis | |style="background:#ABCDEF;" align="center"|'''Function''' || glutamate biosynthesis | ||
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+ | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]''' | ||
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 168 kDa, 5.47 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 168 kDa, 5.47 |
Revision as of 10:35, 17 June 2009
- Description: large subunit of glutamate synthase
Gene name | gltA |
Synonyms | |
Essential | no |
Product | glutamate synthase (large subunit) |
Function | glutamate biosynthesis |
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate | |
MW, pI | 168 kDa, 5.47 |
Gene length, protein length | 4560 bp, 1520 amino acids |
Immediate neighbours | gltC, gltB |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU18450
Phenotypes of a mutant
auxotrophic for glutamate
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH (according to Swiss-Prot) 2 L-glutamate + NADP(+) <=> L-glutamine + 2-oxoglutarate + NADPH
- Protein family: glutamate synthase family (according to Swiss-Prot) glutamate synthase family
- Paralogous protein(s): YerD
Extended information on the protein
- Kinetic information:
- Domains:
- Glutamine amidotransferase type-2 domain (22-415)
- Nucleotide binding domain (1060-1112)
- Modification:
- Cofactor(s): 3Fe-4S, FAD, FMN
- Effectors of protein activity:
- Interactions:
- Localization: membrane associated PubMed, cytoplasm
Database entries
- Structure:
- Swiss prot entry: P39812
- KEGG entry: [3]
- E.C. number: 1.4.1.13 3 1.4.1.13]
Additional information
subject to Clp-dependent proteolysis upon glucose starvation PubMed
Expression and regulation
- Regulation: expression activated by glucose (11 fold) PubMed, induced by sugar PubMed, repressed by arginine PubMed, ammonium required PubMed
- Regulatory mechanism: Activator: GltC PubMed; Repressor: TnrA PubMed, pos. regulated by a mutant form of GltR PubMed
- Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed
Biological materials
- Mutant: GP807 (del gltAB::tet), GP222 (gltA under the control of p-xyl), available in Stülke lab
- Expression vector:
- GFP fusion:
- Antibody:
Labs working on this gene/protein
Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
Jörg Stülke, University of Göttingen, Germany Homepage
Your additional remarks
References