RecO

• Description: mediator of RecA binding to ssDNA, required for the formation of RecA DNA repair centers, , required for efficient survival and replication restart after replication-transcription conflicts
  
  

Gene name	recO
Synonyms	yqxN, yqfI
Essential	no
Product	mediator of RecA binding to ssDNA
Function	DNA repair/ recombination
Gene expression levels in SubtiExpress: recO
Interactions involving this protein in SubtInteract: RecO
MW, pI	29 kDa, 8.208
Gene length, protein length	765 bp, 255 aa
Immediate neighbours	glyQ, yqzL
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

The gene

Basic information

• Locus tag: BSU25280

Phenotypes of a mutant

• drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) PubMed
• recO recJ double mutants are extremely sensitive against DNA damaging agents PubMed
• recO addA-addB double mutants are extremely sensitive against DNA damaging agents PubMed

Database entries

• BsubCyc: BSU25280

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • provides RecA access to ssDNA during chromosomal transformation (together with DprA) PubMed
  • catalyzes annealing of SsbA or SsbA/SsbB coated ssDNAs to allow the formation of DNA duplexes with tails during plasmid transformation PubMed
  • required for the formation of RecA DNA repair centers (together with RecR) PubMed

• Protein family: recO family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SsbA (bound to ssDNA) -RecO, this helps to dislodge SsbA and SsbB from ssDNA PubMed
  • RecR-RecO, RecR:RecO molecular ratio of 2:1 (as judged from other bacteria such as E. coli and Deinococcus radiodurans) PubMed

• Localization:
  • cytoplasm (according to Swiss-Prot)
  • localizes to the DNA entry pole during transformation PubMed

Database entries

• BsubCyc: BSU25280

• Structure:
  • 2V1C (the RecR-RecO complex from Deinococcus radiodurans, RecR: 52% identity, 78% similarity, RecO: 30%/ 58%) PubMed

• UniProt: P42095

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: recO PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A892 ( recO::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: